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文献和实验1 50ml Solution lll 20m 1 50m 1 2x50m 1 Wash Solution (c) 22m 1 2x22m 1 5x22m 1 TE(d) 5m 1 10m 1 40m 1 3S Column 50支 100支 250支 Co11ection tube 50
to microfuge tube, vortex, and ice for another 10min.) 6) Spin at 14000rpm (~16000xg) for 10min at 4OC. 7) Transfer supernatant to new, pre-chilled microfuge tube. 8) Use 5-10 μl for protein assay, or mix 20 μl with 20 μl 2X sample buffer (or 30 μl
Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
8‐Isoprostane EIA kit (8‐iso‐PGF 2α ; Cayman Chemical) containing: Precoated (mouse anti‐rabbit IgG) EIA 96‐well strip plates
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