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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
information Moreover, as this procedure is quite complex, the use of commercial kits such as ApoTagTM (Oncor, Gaithersburg, MD, USA) and "In situ cell death detection Kit" (Boeringer-Mannheim, Germany), is recommended. Utilizing
Materials For purifying plasmid DNA from Escherichia coli cells, the Qiagen Spin Miniprep Kit produces quite reliable results. Do not autoclave solutions containing isopropanol or MOPS; use sterile filtration if necessary.
The Detection of Specific DNA Sequences by Enhanced Chemiluminescence
mutations (Chapter 30 ). In addition, the method of choice for identifying host bacteria containing recombinant DNA sequences continues to be colony or plaque screening using membrane discs.
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