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- 文献和实验
- 技术资料
- CAS号：
529-55-5
- 规格：
1mLx10mM(inDMSO)/1mg/5mg/10mg/25mg/50mg/100mg/200mg
| 规格： | 1mLx10mM(inDMSO) | 产品价格： | ￥2128.0 |
|---|---|---|---|
| 规格： | 1mg | 产品价格： | ￥796.0 |
| 规格： | 5mg | 产品价格： | ￥2184.0 |
| 规格： | 10mg | 产品价格： | ￥3176.0 |
| 规格： | 25mg | 产品价格： | ￥5224.0 |
| 规格： | 50mg | 产品价格： | ￥7112.0 |
| 规格： | 100mg | 产品价格： | ￥9440.0 |
| 规格： | 200mg | 产品价格： | ￥12720.0 |
Product Introduction
Bioactivity
| 名称 | Prunin |
| 描述 | Prunin (Naringenin 7-0-glucoside) possesses anti-diabetic, and anti-abacterial properties, it can inhibit protein tyrosine phosphatase 1B (PTP1B) and stimulate glucose uptake in insulin-resistant HepG2 cells; it also can stimulate growth of Pseudomonas aeruginosa and different Bacilllus sp. Prunin exhibits a markedly enhanced solubility compared to naringenin and naringin while maintaining the in vitro inhibition of HMG-CoA reductase. |
| 体外活性 | Prunin与HG-烷基（C4-C18）酯（0.1-100μM）对人类白血病T（Jurkat）细胞活性及其质膜流动性的影响进行了评估。在此浓度下，还观察到细胞超极化和收缩现象。然而，细胞质膜流动性未受影响，无论评估的膜层深度如何，但发现质膜水合作用有轻微变化。酯化作用并未影响Prunin和HG（0.1-10μM）对1mM H2O2的抗氧化能力。当暴露于1mM AAPH时，P-烷基酯保持了Prunin的抗氧化能力，但HG衍生物表现为促氧化剂[1]。 |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 2 mg/mL (4.6 mM), Sonication is recommended. DMSO : 25 mg/mL (57.55 mM), Sonication is recommended. |
| 关键字 | VirusProtease | Virus Protease | synthesis | RNA | Rhinovirus | Prunin | Phosphatase | Inhibitor | inhibit | infection | HRVs | HRV | HMG-CoA reductase | HEVs | HEV | Enterovirus | Anti-infection | Antiinfection | Antifection | anti-diabetic |
| 相关产品 | Hexane-1,6-diol | Sodium orthovanadate | L-Lysine hydrochloride | Penicillin G potassium | Vidarabine | Stearic acid | Ofloxacin | (E)-3-(p-Tolyl)acrylaldehyde | L-Ascorbic acid 2-phosphate magnesium | L-Lysine | Thymidine | Phenytoin sodium |
| 相关库 | Inhibitor Library | Bioactive Compound Library | Bioactive Compounds Library Max | Ancient Chinese Classical Formulas Compound Library | Flavonoid Natural Product Library | Food as Medicine Compound Library | Flavor Natural Product library | Natural Product Library | Anti-Viral Compound Library | Anti-virus Traditional Chinese Medicine Monomer Library | Polyphenolic Natural Product Library | Anti-infective Natural Product Library |
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
(dip 3-6 times or leave ~15-60 seconds). The section should not be too dark. The methyl green concentration can be varied if sections are generally too dark or too light. It's better to start with a lower concentration. dH2O 1-5 mins
Polymerase Chain Reaction (PCR) to Amplify rRNA Gene Fragment
), close tube tightly, place in thermal cycler. Initiate thermal cycling program. PCR55 Phase 1 - 1 cycle Initial denaturation 4 min. @ 94o C Primer annealing 45 sec. @ 55o C Primer extension2
CHAPS Insoluble Floating Fraction Preparation
and then stored frozen at -70o . Version 2: Sucrose crystals (Sigma) were added to the CHAPS lysate to a final concentration of 55% (w/v). A 8 ml linear 20-45% sucrose gradient (in TEBP) was formed over 4 mls of this sucrose-containing CHAPS lysate
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