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- 文献和实验
- 技术资料
- CAS号:
75-80-9
- 规格:
1mLx10mM(inDMSO)/1g/5g/100mg/500mg
| 规格: | 1mLx10mM(inDMSO) | 产品价格: | ¥168.0 |
|---|---|---|---|
| 规格: | 1g | 产品价格: | ¥332.0 |
| 规格: | 5g | 产品价格: | ¥788.0 |
| 规格: | 100mg | 产品价格: | ¥132.0 |
| 规格: | 500mg | 产品价格: | ¥253.0 |
Product Introduction
Bioactivity
| 名称 | Tribromoethyl alcohol |
| 描述 | Tribromoethyl alcohol (Avertin) has anesthetic activity and is often used as an anesthetic in experimental animals, especially rodents. Tribromoethyl alcohol has a short duration of anesthesia and a rapid onset of anesthesia. This product also provides a ready to use version without the need for preparation, with better stability and repeatability. Ready To Use Tribromoethanol: C0183 1.25% Avertin (Anesthesia For Mice) and C0184 2.5% Avertin (Anesthesia For Rat). |
| 体内活性 | 方法:为测试麻醉效果,将 Tribromoethyl alcohol (125-500 mg/kg) 腹腔注射给 ICR 小鼠。 结果:所有剂量的 Tribromoethyl alcohol 都能诱导稳定的麻醉,并且麻醉时间保持呈剂量依赖性。在高剂量组中,雌性小鼠的麻醉时间比雄性小鼠长,雌性小鼠麻醉的持续时间明显长于雄性小鼠。在中剂量组和高剂量组中,雌性小鼠的恢复时间明显长于雄性小鼠。[1] |
| 存储条件 | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
| 溶解度 | DMSO : 237 mg/mL (838.17 mM), Sonication is recommended. H2O : 40 mg/mL (141.46 mM), Sonication is recommended. 10% DMSO+40% PEG300+5% Tween 80+45% Saline : 5 mg/mL (17.68 mM), Sonication is recommended. Ethanol : 52 mg/mL (183.9 mM), Sonication is recommended. |
| 关键字 | Tribromoethyl alcohol | Narcolan | Inhibitor | inhibit |
| 相关库 | CNS-Penetrant Compound Library | Bioactive Compound Library | Bioactive Compounds Library Max | NO PAINS Compound Library |
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文献和实验的FCS,1%青霉素/链霉素,B16F10细胞和RPMI 10%FCS的 1%青霉素/链霉素,L1C2细胞在4°C保存。 试剂的准备: 1.准备上述所需细胞培养试剂,在37°C预热。时间5分钟。 解冻肿瘤细胞: 2. 将细胞从液氮中拿出,并进行溶解解冻。 3. 完全解冻后,细胞离心800rpm 7分钟,4°C。 4.弃上清用10ml液体悬浮细胞。 5.在50毫升培养瓶中培养细胞1-2天,直到细胞达到70-80%融合
Procedure for Transfection of Mammalian Cells
in a CO2 incubator until the cells are 70-80% confluent. This will usually take 18-24 h. Prepare the following solutions in 12 x 75 mm sterile tubes: Solution A: For each transfection, dilute 2 μg DNA (plasmid) in 375 μl serum-free IMDM
the pellet. Add 400 ul 75% EtOH, vortex briefly. Spin at 4°C, 4000 rpm for 10 min. Remove s/n with pipet and invert racked tubes in hood with door half shut. Approx. 10-15 minutes; do not overdry. Add 300 l DEPC-treated ddH2 O
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