- ￥2980 - 6880
- Sino Biological
- 北京
- 30081-H08H-D
- 2026年06月04日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Human CA125/MUC16 Protein (AF 488 Conjugation, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
25.00 µg/100.00 µg
| 规格： | 25.00 µg | 产品价格： | ￥2980.0 |
|---|---|---|---|
| 规格： | 100.00 µg | 产品价格： | ￥6880.0 |
蛋白名称：Human CA125/MUC16 Protein (AF 488 Conjugation, His Tag)
蛋白构建：A DNA sequence encoding the human CA125 (Q8WXI7-1) (Pro13810-Pro14451) was expressed with a polyhistidine tag at the C-terminus. are conjugated with AF 488 under optimum conditions.
表达宿主：HEK293 Cells
蛋白活性：1.Flow cytometric analysis of anti-MUC16 CAR expression. 5e5 of anti-MUC16 CAR-293 cells were stained with 100 μL of 10 μg/mL of AF 488-conjugated human CA125/MUC16 Protein (AF 488 Conjugation, His Tag, Cat. No. 30081-H08H-D) and negative control protein. Non-transduced 293 cells were used as a control (left). AF 488 signal was used to evaluate the binding activity (QC tested). 2.Binding activity of AF 488-conjugated MUC16 protein to PBMC cells. PBMC cells were stained with PE conjugated anti-CD3 antibody and AF 488-conjugated human CA125/MUC16 Protein (AF 488 Conjugation, His Tag, Cat. No. 30081-H08H-D) and detected by flow cytometry. PE signal was used to evaluate the content of CD3+ T cells in PBMCs. AF 488 signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Pro 13810
蛋白分子量：The recombinant Human CA125/MUC16 comprises 653 amino acids and has a predicted molecular mass of 73.27 kDa.
蛋白NP号：Q8WXI7-1
蛋白氨基酸序列：Pro13810-Pro14451
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文献和实验His 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面： 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螯合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲液的pH、盐
flow field-flow fractionation (AF4) conditions that have proven their reliability for the analysis of quantum dots and other nanoparticles in the 5–50 nm size range. Common pitfalls are emphasized together with strategies to overcome them.
Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
Low (“Low”)Imidazole Buffer 0.5L 100mM Imidizole 3.4g 5% glycerol 25ml 100% glycerol 50mM Tris-HCl (pH 7.9)50ml 0.5M Tris-HCl (pH 7.9) 0.1% Tween-20 0.5M 100% Tween-20 500mM NaCl50ml 5M NaCl dH2O Fill to 0.5L (start w/ 350ml) High (“High
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