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Recombinant GSK3 beta Antibody

,PBS Only,见COA,阿拉丁
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  • ¥1199.90 - 32999.90
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  • Ab335948
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  • 2026年06月01日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 库存

      期货

    • 英文名

      Recombinant GSK3 beta Antibody

    • 供应商

      上海阿拉丁生化科技股份有限公司

    • 保存条件

      -20°C储存,避免反复冻融;超低温运输

    • 规格

      Ab335948-100μg/Ab335948-10μg/Ab335948-1mg/Ab335948-50μg

    规格:Ab335948-100μg产品价格:¥5499.90
    规格:Ab335948-10μg产品价格:¥1199.90
    规格:Ab335948-1mg产品价格:¥32999.90
    规格:Ab335948-50μg产品价格:¥3499.90

    基本信息

    产品名称:Recombinant GSK3 beta Antibody

    规格或纯度:PBS Only,见COA

    应用:WB, IF/ICC, IHC

    特异性:GSK3 beta

    种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat), 仓鼠(Hamster)

    免疫原:A synthetic peptide derived from human GSK3 beta (AA 1-13)

    阳性对照:WB: Jurkat, HeLa, HEK293, A431, NIH/3T3 and C6 cell lysates. IHC: Human cholangiocarcinoma. IF/ICC: HeLa cells.

    偶联:Unconjugated


    产品属性

    克隆类型:重组抗体

    Format:Whole IgG

    轻链亚型:kappa

    SDS-PAGE:150 kDa

    纯化方法:Protein A purified

    物理外观:Liquid

    储存缓冲液:PBS, pH 7.4

    防腐剂:No

    浓度:见COA

    储存条件:-20°C储存,避免反复冻融

    运输条件:超低温运输

    稳定性与储存:4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。



    功能和特点

    宿主种属:兔(Rabbit)

    种属反应性:人(Human), 小鼠(Mouse), 大鼠(Rat), 仓鼠(Hamster)

    偶联:Unconjugated

    图片

    产品细节图片1>
    Western Blot: Recombinant GSK3 beta Antibody (Ab106628)

    Recombinant GSK3 beta Antibody (Ab106628) - Western Blot
    All lanes: Recombinant GSK3 beta Antibody (Ab106628) at 1/1000 dilution
    Samples: Lysates at 20 µg per lane
    Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

    Predicted band size: 47 kDa
    Observed band size: 45 kDa
    Exposure time: 10.7 s

    产品细节图片2>
    IHC: Recombinant GSK3 beta Antibody (Ab106628)

    Recombinant GSK3 beta Antibody (Ab106628) - IHC
    Immunohistochemistry analysis of paraffin-embedded Human Cholangiocarcinoma using Recombinant GSK3 beta Antibody (Ab106628). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

    产品细节图片3>
    IF/ICC: Recombinant GSK3 beta Antibody (Ab106628)
    IF/ICC: Recombinant GSK3 beta Antibody (Ab106628)
    IF analysis of Recombinant GSK3 beta Antibody (Ab106628) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant GSK3 beta Antibody (Ab106628) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

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    图标文献和实验
    相关实验
    • v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平

      are prepared using Adobe Photoshop and MicrosoftPowerpoint software. 2.5. Immunoprecipitation Kinase Activity Assays 1. The anti-GSK3b monoclonal antibody is from BD TransductionLaboratories (#610201) and protein G-Sepharose is fromAmersham (#17-0618-01

    • secondary antibody review -- data from 99 publications

      cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

    • v468 chapter 5 糖原合成酶激酶-3 的抑制

      synthase kinase 3beta as a target for the therapy of shock and inflammation. Shock. 27 , 113–123. 8. Rubinfeld B, Albert I, Porfiri E, Fiol C, Munemitsu S, Polakis P. (1996) Binding of GSK3beta to the APC-beta-catenin complex

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