AV38156-100UL默克西格玛Anti-MyC ant

IMMUNOSCREENING OF WITH ANTIBODY PROBES

Adsroption of Anti-E. coli Antibodies Grow E. coli strain Y1090 in L broth at 37°C overnight. Transfer to 10 mM MgSO4 and store at 4°C for future use. These cells are good for about 1 week. Soak one 132 mm nitrocellulose membrane per Petri dish

secondary antibody review -- data from 99 publications

cytometry   used as a control to detect cell responses targeted antigen   7       Alexa Fluor 488         7       Cy3         8 goat IgG   Alexa Fluor 488   1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

To immunostain using enzyme-coupled secondary antibodies: i. Rinse slides in PBS and remove excess solution. ii. Add 40 µL of secondary antibody (e.g., anti-rabbit IgG [Fc] HRP conjugate, 1:100 dilution