MAP2 Polyclonal antibody

【产品概述】
本产品为MAP2 Polyclonal antibody（货号：17490-1-AP），由Rabbit / IgG宿主制备。该抗体属于Polyclonal抗体，可识别human, mouse, rat来源的靶蛋白，经测试适用于WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, ELISA等实验应用。产品形式为Liquid，偶联物类型为Unconjugated。

【背景介绍】
Microtubule-associated protein 2 (MAP2) is a tubulin binding protein regulating the spacing and stability of microtubules and contributing to elongation of dendrites. What is the molecular weight of MAP2? Is MAP2 post-translationally modified? MAP2 has multiple isoforms that arise from alternative splicing (PMID: 3121794, 7854050, and 10383434). They are classified into two groups - MAP2A and MAP2B, which are known as high molecular weight (HMW) isoforms, run as ~280 kDa species, while low molecular weight (LMW) isoforms MAP2C and MAP2D are around ~70 kDa. MAP2 proteins are heavily phosphorylated, which contributes to a large discrepancy between their predicted and observed molecular weight in SDS-PAGE (220 vs 280 kDa for HMW forms). What is the tissue expression pattern of MAP2? What is the subcellular localization of MAP2? MAP2 isoforms differ in their tissue and developmental expression pattern (PMID: 2469170 and 3898077). In the brain, MAP2B is widely expressed during and post development, MAP2A is expressed postnatally, while MAP2C is present only in the early development except of present in photosensitive cells of the adult retina and in the olfactory system. MAP proteins are highly expressed in the CNS found in cell bodies and dendrites of neurons, in dorsal root ganglion, reactive glia, and in the testis (PMID: 9588626). In neurons, MAP2 proteins are found in the cell body and dendrites, where they associate with microtubules, while they can also be present in the nuclei of testicular cells. Can MAP2 be used as a neuronal marker? MAP2 proteins are abundantly expressed in neurons. MAP2 is frequently used as a dendritic marker because it is present in the cell body and dendrites of neurons but absent in axons (PMID: 28413822).

【产品特点】
本抗体采用Antigen affinity purification方式纯化，确保了高特异性和批次间一致性。观测分子量约为280 kDa, 70-85 kDa。推荐稀释比：WB: WB : 1:5000-1:50000；IHC: IHC : 1:2500-1:10000；IP: IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate。Western Blot检测阳性对照组织/细胞为SH-SY5Y cells, rat brain tissue, mouse brain tissue。储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.。Proteintech抗体经过严格的质量验证流程，确保研究人员获得可重复且可靠的实验结果。

【产品信息】
GenBank蛋白编号：BC038857；基因名称：MAP2；Gene ID (NCBI)：4133；UNIPROT ID：P11137；RRID：AB_2137880；别名包括：MAP 2, MAP-2, MAP2A, MAP2B, MAP2C；免疫原为CatNo:Ag11580Product name: Recombinant human MAP2 proteinSource:e coli.-derived, PGEX-4TTag: GSTDomain: 213-559 aa ofBC038857Sequence: TSAGSTDRLPYSKSGNKDGVTKSPEKRSSLPRPSSILPPRRGVSGDRDENSFSLNSSISSSARRTTRSEPIRRAGKSGTSTPTTPGSTAITPGTPPSYSSRTPGTPGTPSYPRTPHTPGTPKSAILVPSEKKVAIIRTPPKSPATPKQLRLINQPLPDLKNVKSKIGSTDNIKYQPKGGQVRILNKKIDFSKVQSRCGSKDNIKHSAGGGNVQIVTKKIDLSHVTSKCGSLKNIRHRPGGGRVKIESVKLDFKEKAQAKVGSLDNAHHVPGGGNVKIDSQKLNFREHAKARVDHGAEIITQSPGRSSVASPRRLSNVSSSGSINLLESPQLATLAEDVTAALAKQGL种属同源性预测；储存缓冲液为PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.，储存条件为Store at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.；产品形式为Liquid；纯化方式为Antigen affinity purification。