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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
3 months at -20°C
- 英文名:
Shh (C25II), Mouse
- 库存:
现货
- 供应商:
上海经科化学科技有限公司
- 规格:
10μg/50μg/1mg
| 规格: | 10μg | 产品价格: | ¥500.0 |
|---|---|---|---|
| 规格: | 50μg | 产品价格: | ¥1200.0 |
| 规格: | 1mg | 产品价格: | ¥9600.0 |
| Species | Mouse | |
| Protein Construction |
|
|
| Purity | > 95% as analyzed by SDS-PAGE > 95% as analyzed by HPLC |
|
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | |
| Biological Activity | ED50 < 2.0 μg/ml, measured by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) Cells, corresponding to a specific activity of > 500.0 U/mg. | |
| Expression System | E. coli | |
| Apparent Molecular Weight | ~19.7 kDa, on SDS-PAGE under reducing conditions. | |
| Formulation | Lyophilized after extensive dialysis against PBS. | |
| Reconstitution | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. | |
| Storage & Stability | Upon receiving, this product remains stable for 6 months at lower than -70°C. Upon reconstitution, the product should be stable for 1 week at 4°C or for 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background | Sonic Hedgehog (Shh) is a member of the Hedgehog (Hh) family of highly conserved proteins which are widely represented throughout the animal kingdom. In mammal, there are three related Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh). They share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). Sonic Hedgehog plays a role in cell growth, cell specialization, and the normal shaping (patterning) of the body. Shh is also important for development of the brain and spinal cord (central nervous system), eyes, limbs, and many other parts of the body. |
| Synonyms | Hhg1; HHG-1; ShhNC; Sonic hedgehog protein N-product; Sonic hedgehog protein 19 kDa product |
产品特点:
1. 【小鼠源天然序列】采用小鼠 FGF acidic 天然氨基酸序列,无突变,与小鼠体内天然蛋白结构完全一致,适配小鼠来源细胞的实验需求,无种属差异2. 【高活性高特异性】可高效结合 FGFR 受体,ED₅₀ < 100 ng/mL(受体结合实验),可被 FGF acidic 特异性抗体识别,适用于细胞培养、抗体筛选3. 【高纯度低内毒素】SDS-PAGE 纯度 > 95%,内毒素 < 0.2 EU/μg(LAL 法),适配细胞培养、体内动物实验等敏感场景需求4. 【易纯化检测】哺乳动物细胞表达,可通过亲和层析快速纯化,也可通过 FGF acidic 特异性抗体进行检测5. 【稳定易保存】蛋白储存液配方优化,未开封 - 80℃可稳定保存 12 个月,-20℃可存 6 个月,避免反复冻融可长期保持蛋白活性6. 【多场景适配】可用于 FGF acidic 蛋白结构与功能研究、细胞培养添加剂、肿瘤机制研究、抗体制备与筛选、药物靶点验证等
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缺失的小鼠模型。ApoE小鼠模型表现出异常高血脂症状,在3月龄时即出现动脉脂肪堆积;17月龄时小鼠脑内将出现脂瘤性纤维瘤;学习记忆能力出现障碍;其与CHD、高脂血症、脑梗塞、AD及慢性乙型肝炎等疾病相关 应用: 一般用APOE鼠进行动脉粥样硬化的药效学研究。 03、BALB/c品系 来源: 1913年,贝格(Bagg)从美国商人欧希尔(Ohio)处购得的白化小鼠原种,以群内方法繁殖。麦克·多威尔(MacDowell)在1923年开始作近交
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