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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
BODIPY-FL NHS ester
- 库存:
现货
- CAS号:
146616-66-2
- 规格:
1 mg/5 mg/10 mg/25 mg
| 规格: | 1 mg | 产品价格: | ¥480.0 |
|---|---|---|---|
| 规格: | 5 mg | 产品价格: | ¥940.0 |
| 规格: | 10 mg | 产品价格: | ¥1520.0 |
| 规格: | 25 mg | 产品价格: | ¥3040.0 |
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文献和实验----------------------- Screening for Protein-Protein Interactions. Protein G was from Pierce (Rockford, IL) and BODIPY-FL-Goat-anti-Mouse IgG was from Molecular Probes (Eugene, OR). IKBalpha and p50 were kindly provided by T. Maniatis (Harvard University, Cambridge, MA
. The DNA strand nicks characteristic of apoptosis were detected with the TUNEL (terminal deoxynucleotidyl transferase–mediated dUTP nick end-labeling) assay using the fluorescently labeled nucleotide, ChromaTide BODIPY FL-14-dUTP (C7614). Image contributed
Coupling Peptides To Resin For Affinity Purification
5% ethylene diamine in water. Incubate 15' at RT. Wash with 10 volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate
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