mHGF

Hepatocyte  Growth  Factor(HGF)  ,  also  known   as  Scatter Factor and Hepatopoietin A, is a pleiotropic  protein in the Plasminogen subfamily of S1 peptidases. It   is   a   multidomain   molecule   that   includes   an N-terminal   PAN/APPLE-like   domain,four   Kringle domains, and a serine proteinase-like domain that has no   detectable   protease   activity.   Mouse   HGF   is secreted  as  an  inactive  728  amino  acid  (aa)  single chain propeptide. It is cleaved after the fourth Kringle domain   by   a   serine   protease   to   form   bioactive disulfide-linked HGF with a 60 kDa alpha     and  30 kDa  beta       chain.  Alternate  splicing  generates  an isoform that lacks the peptidase and the second, third, and fourth Kringle domains. Mouse HGF shares 91% - 95% aa sequence identity with bovine,canine, feline, human,  and  rat  HGF.  HGF  binds  heparan-sulfate proteoglycans   and   the   widely   expressed   receptor tyrosine    kinase,     HGF    R/c-MET.HGF-dependent c-MET activation is implicated in the development of many   human    cancers.   HGF   regulates   epithelial morphogenesis   by    inducing    cell    scattering   and branching     tubulogenesis.     HGF      induces     the up-regulation of integrin alpha 2 beta  1 in epithelial cells   by   a   selective   increase   in   alpha   2    gene transcription.  This   integrin  serves  as  a  collagen  I receptor,  and  its  blockade  disrupts  epithelial  cell branching    tubulogenesis.    HGF    can    also    alter epithelium morphology by the induction of nectin-1 alpha   ectodomain   shedding,   an   adhesion   protein component of adherens junctions. In the thyroid, HGF induces   the   proliferation,   motility,    and   loss    of differentiation  markers   of  thyrocytes   and   inhibits TSH-stimulated iodine uptake.

基本信息：

别 名 (synonym) ：F-TCF; hepatocyte  growth factor (hepapoietinA;    scatter     factor);    Hepatopoeitin-A; Hepatopoietin A; HGF

来源(Source)：Human embryonic kidney cell, HEK293-derived mouse HGF protein

蛋白结构（Structure):该蛋白不含标签 

基因 ID：Q08048

氨基酸序列：Gln33-Arg495 (alpha) & Val496-Leu728 (beta)

分子量大小(MW)：79.3 KDa

纯度（Purity）：> 95%, determined by SDS-PAGE

 

制剂(Formulation)：

Solution protein.Dissolved in sterile PBS buffer.This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.

 

产品数据：

 

1.SDS-PAGE

 

2 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and visualized by Coomassie Blue staining.

 

2.生物活性（Bioactivity）

 

Recombinant mouse HGF stimulates cell proliferation of the mIMCD-3 mouse epithelial cells.

 

订购信息：

 

货号	级别	规格	牌价/￥
CY145F0010L	RUO	10μg	880
CY145F0100L	RUO	100μg	3630
CY145F1000L	RUO	1000μg	18480