TG100-115

TG100-115 effectively targets PI3Kγ and PI3Kδ, with IC50s of 83 nM and 235 nM, respectively, while showing minimal impact on PI3Kα and PI3Kβ (IC50 values >1 μM). In terms of specificity, TG100-115 was tested against a panel of 133 protein kinases and did not inhibit any at IC50 values <1 μM. TG100-115 strongly counteracts edema and inflammation, which are key factors in myocardial infarction, by inhibiting mediators like vascular endothelial growth factor and platelet-activating factor. Conversely, it does not affect endothelial cell mitogenesis, which is crucial for tissue repair following ischemic damage¹.

溶解方案(细胞实验)

DMSO 中的溶解度 : 33.33 mg/mL (96.23 mM; ultrasonic and warming and adjust pH to 2 with HCl and heat to 60°C; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

溶解方案(动物实验)

"方案 一": "请依序添加每种溶剂：10% DMSO 40% PEG300 5% Tween-80 45% SalineSolubility: 2.5 mg/mL (7.22 mM); 澄清溶液; 超声助溶 此方案可获得 2.5 mg/mL的澄清溶液。以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；再向上述体系中加入 50 μL Tween-80，混合均匀；然后再继续加入 450 μL 生理盐水 定容至 1 mL。生理盐水的配制：将 0.9 g 氯化钠，溶解于 ddH₂O 并定容至 100 mL，可以得到澄清透明的生理盐水溶液。"

"方案 二": "请依序添加每种溶剂：10% DMSO 90% (20% SBE-β-CD in Saline)Solubility: 2.5 mg/mL (7.22 mM); 澄清溶液; 超声助溶 此方案可获得 2.5 mg/mL的澄清溶液。以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液 中，混合均匀。2 g SBE-β-CD（磺丁基醚 β-环糊精）粉末定容于 10 mL 的生理盐水中，完全溶解至澄清透明。"