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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.065 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人γ-氨基丁酸B受体关联蛋白(gABARBP)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human GABARBP(GABA-Receptor B Associated Protein ) Microsample ELISA Kit |
| 别名 | GABARBP |
| 货号 | ELK8832MS |
| 反应种属 | Human |
| 检测类型 | Sandwich |
| 灵敏度 | 0.065 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GABARBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GABARBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GABARBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GABARBP in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |

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文献和实验GABA (γ-aminobutyric acid) receptors are among the most ubiquitous in the brain, having been identified (neurophysiologitally) in all regions and at all levels (e.g., Iversen, 1978 ). All neuronal cells respond to the iontophoretic application
Senescent-associated b-Gal Assay
Wash cells with PBS Fix cells in 0.2% glutaraldehyde for 5’ dilute this in PBS Wash cell with PBS Stain with X-gal solution (ingredients listed below) for 6 to 24 hours at 37 °C
Senescent-associated b-Gal Assay
Senescent-associated b -Gal Assay Wash cells with PBS Fix cells in 0.2% glutaraldehyde for 5’ dilute this in PBS Wash cell with PBS Stain with X-gal solution (ingredients listed
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