人铁反应元件结合蛋白2(IREB2)微量上样酶联免疫吸附检测
试剂盒【Human IREB2(Iron Responsive Element Binding Protein 2) Microsample ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.058 ng/mL
- 武汉
- ELK4764MS
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Serum, plasma, tissue homogenates and other biological fluids.
- 灵敏度：
0.058 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人铁反应元件结合蛋白2(IREB2)微量上样酶联免疫吸附检测试剂盒 |
| 英文名称 | Human IREB2(Iron Responsive Element Binding Protein 2) Microsample ELISA Kit |
| 别名 | IRP2; Iron regulatory protein 2 |
| 货号 | ELK4764MS |
| 反应种属 | Human |
| P48200 | P48200 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.058 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IREB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IREB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IREB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IREB2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Signal transduction; |
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文献和实验Purification of Sequence‐Specific DNA‐Binding Proteins by Affinity Chromatography
chromatography is a very effective and straightforward means of purifying a protein based on its sequence?specific DNA?binding properties. The affinity chromatography procedure described in this unit uses DNA containing specific recognition sites for the desired
[the iron responsive element (IRE), AU-rich class-2 element (ARE), selenocysteine insertion sequence (SECIS), etc.] resides in each sequence. The UAlbany TUTR collection is a shared resource that is available to researchers for software development
Regulation of AP-1 Activity by TGF
was found in several 12-O -tetradecanoylphorbol-13-acetate (TPA)-responsive genes and, hence, is also designated a TRE or TPA-responsive element. Heterodimers can form between c-Jun and ATF-2, a member of the CREB (CRE binding) protein family, allowing the complex to bind
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