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- 文献和实验
- 技术资料
- 供应商:
武汉研升生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度:
0.052 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1680.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2400.0 |
| 中文名称 | 人嘌呤能受体P2Y14(P2RY14)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human P2RY14(Purinergic Receptor P2Y, G Protein Coupled 14) ELISA Kit |
| 别名 | GPR105; P2Y14; P2Y Purinoceptor 14; G Protein-Coupled Receptor 105; UDP-glucose receptor |
| 货号 | ELK4565 |
| 反应种属 | Human |
| Q15391 | Q15391 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.052 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human P2RY14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human P2RY14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human P2RY14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human P2RY14 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | CD & Adhesion molecule; |

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文献和实验G-Protein-Coupled Receptor Phosphorylation and Palmitoylation
It is now clear that nearly all G-protein-coupled receptors (GPCRs) are phosphorylated and palmitolyated. The process of receptor phosphorylation has been extensively studied because it offers a regulatory mechanism that is both rapid and dynamic
of neurotransmitters. Adenosine acts as a general depressant in the brain. In contrast, adenosine receptor antagonists show somnolytic and stimulant properties. Although purinergic psychopharmaceuticals are rare, the nonselective adenosine receptor antagonist caffeine
Elucidating Agonist-Selective Mechanisms of G Protein-Coupled Receptor Desensitization
In pharmacology, a central tenet of receptor theory has been that different agonists acting at a particular G protein-coupled receptor subtype produce the same profile of cellular responses. In recent years, advances
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