人细胞色素C氧化酶亚基Ⅳ亚型1(COX4I1)酶联免疫吸附检
测试剂盒【Human COX4I1(Cytochrome C Oxidase Subunit IV Isoform 1) ELISA Kit】- ￥1820 - 2600
- ELK Biotechnology
- 0.16-10 ng/mL
- 0.059 ng/mL
- 武汉
- ELK4396
- 2026年05月21日
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- 文献和实验
- 技术资料
- 供应商：
武汉研升生物科有限公司
- 检测范围：
0.16-10 ng/mL
- 检测方法：
Sandwich
- 适应物种：
Human
- 样本：
Tissue homogenates, cell lysates and other biological fluids.
- 灵敏度：
0.059 ng/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1820.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2600.0 |
| 中文名称 | 人细胞色素C氧化酶亚基Ⅳ亚型1(COX4I1)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human COX4I1(Cytochrome C Oxidase Subunit IV Isoform 1) ELISA Kit |
| 别名 | COX4; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
| 货号 | ELK4396 |
| 反应种属 | Human |
| P13073 | P13073 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.059 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids. |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COX4I1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COX4I1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COX4I1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COX4I1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |
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文献和实验亦称细胞色素氧化酶。在细胞呼吸中处于细胞色素系统的末端。此酶是把呼吸底物的电子经过细胞色素系统直接传递给分子态氧（即具有自动氧化作用）。 EC1． 9． 3． 1。通常用还原型细胞色素 c的氧催化氧化的作用来进行活性测定。凯林（ D． Keilin， 1930）在心肌提取物中首先发现此酶的活性。以前认为是靛酚氧化酶所催化，后来明确了靛酚反应是与细胞色素 c和细胞色素 c氧化酶二者都有关系。从那以后，凯林在心肌和酵母中发现与一氧化碳结合的一种细胞色素（细胞色素 a3 ），并认为
(ubiquinol cytochrome c oxido reductase or bc1 complex), IV (cytochrome c oxidase), and V (ATP synthase). These respiratory complexes are conserved from the yeast Saccharomyces cerevisiae to human with the exception of complex I, which is replaced
Identification of Mutations in mtDNA from Patients Suffering Mitochondrial Diseases
, all of which are subunits of the respiratory chain complexes. Of the 13 structural genes, 7 encode subunits of complex I (NADH-CoQ oxidoreductase), 1 encodes the cytochrome-b subunit of complex III (CoQ-cytochrome-c oxidoreductase), 3 encode subunits of complex IV
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