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- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.63-40 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Rat
- 样本:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 灵敏度:
0.36 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)酶联免疫吸附检测试剂盒 |
| 英文名称 | Rat TRACP-5b(TartrateResistant Acid Phosphatase 5b) ELISA Kit |
| 别名 | ACP5;TRAP5b;SPENCDI |
| 货号 | ELK9632 |
| 反应种属 | Rat |
| 检测类型 | Sandwich |
| 灵敏度 | 0.36 ng/mL |
| 标准品 | 40 ng/mL |
| 检测范围 | 0.63-40 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Immunology;Cardiovascular |

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文献和实验大鼠抗酒石酸酸性磷酸酶5b(TRACP-5b)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠抗酒石酸酸性磷酸酶 5b(TRACP-5b)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 TRACP-5b 单抗包被于酶标板上,标准品和样品中的 TRACP-5b 与单抗结合,加入生物素化的抗大鼠
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Nenonen, A., Cheng, S., Ivaska, K.K., Alatalo, S.L., Lehtimaki, T., Schmidt‐Gayk, H., Uusi‐Rasi, K., Heinonen, A., Kannus, P., Sievanen, H., Vuori, I., Vaananen, H.K., and Halleen, J.M. 2005. Serum TRACP 5b is a useful marker
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