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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Serum, plasma, tissue homogenates and other biological fluids
- 灵敏度:
0.063 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人同源域相互作用蛋白激酶2(HIPK2)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human HIPK2(Homeodomain Interacting Protein Kinase 2) ELISA Kit |
| 别名 | hHIPk 2; hHIPk2; HIPK 2; Hipk2; |
| 货号 | ELK5068 |
| 反应种属 | Human |
| Q9H2X6 | Q9H2X6 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.063 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Serum, plasma, tissue homogenates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HIPK2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HIPK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HIPK2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HIPK2 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Enzyme & Kinase; |

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文献和实验Interaction Trap/Two‐Hybrid System to Identify Interacting Proteins
pentagon, bait protein; open rectangle, noninteracting library protein; shaded box, activator protein (acid blob in Fig. ); open chevron, interacting library protein. View Image
相关专题 随着生物信息学、蛋白组学的发展,研究蛋白结构域,蛋白序列等研究越来越成为必备技能,因此,蛋白数据库的了解和使用越来越显得重要。对此,北京大学 医学部就列出了各个蛋白相关数据库的链接,方便科研人员研究和使用。 通用数据库 MIPS - Protein sequences and complete genomic sequences
://www.allgenes.org/ Human and mouse gene index integrating gene, transcript and protein annotation 38. Ares Lab Yeast Intron Database http://www.cse.ucsc.edu/research/compbio/yeast_introns.htmlyeast_introns.html Splicesomal introns in Saccharomyces
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