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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
武汉益普生物科有限公司
- 检测范围:
0.16-10 ng/mL
- 检测方法:
Sandwich
- 适应物种:
Human
- 样本:
Tissue homogenates, cell lysates and other biological fluids
- 灵敏度:
0.061 ng/mL
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1820.0 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥2600.0 |
| 中文名称 | 人Toll相互作用蛋白(TOLLIP)酶联免疫吸附检测试剂盒 |
| 英文名称 | Human TOLLIP(Toll Interacting Protein) ELISA Kit |
| 别名 | IL-1RAcPIP |
| 货号 | ELK4871 |
| 反应种属 | Human |
| Q9H0E2 | Q9H0E2 |
| 检测类型 | Sandwich |
| 灵敏度 | 0.061 ng/mL |
| 标准品 | 10 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | Tissue homogenates, cell lysates and other biological fluids |
| 反应时间 | 3.5h |
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TOLLIP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TOLLIP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TOLLIP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TOLLIP in the samples is then determined by comparing the OD of the samples to the standard curve. |
| 研究领域 | Infection immunity; |

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文献和实验的原理用于分子诊断方面:TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的,相关文章见:1,Rapid screen for truncating ATM mutations by PTT-ELISA . 2,An ELISA-based high throughput protein truncation test
认为,蛋白质首先通过疏水相互作用结合到NC膜上,通过静电力牢固地与NC膜结合则。该结合模式同大量已发表的文献结果一致,然而静电作用机理对于采用干燥或乙醇吸附方法到达的蛋白质长期稳定的吸附于NC膜上无法提高合理的解释。不管蛋白质结合的作用力如何达到平衡,研究人员在优化蛋白质吸附于特定的NC膜时,必须综合考虑所有影响蛋白质吸附的作用力。这一观点不可避免的影响着试纸条板材的选择及其处理工艺。例如,如果产品开发人员选用可以极度降低静电相互作用或者疏水相互作用的缓冲液,那么蛋白质的吸附能力则剧烈的降低。同理
产品说明 规格 价格 (RMB) 100.01 100.02 Dynabeads Protein A 1ml Dynabeads Protein A can bind 250 human IgG. 1 ml 5 ml 3900 8255
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