- ￥5170
- Sino Biological
- 北京
- 41056-V27H-B
- 2026年04月20日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Influenza A H3N2 (A/District of Columbia/27/2023) Hemagglutinin / HA-specific B cell probe Protein Trimer (AVI & His Tag), Biotinylated (MALS-verified)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg
蛋白名称：Influenza A H3N2 (A/District of Columbia/27/2023) Hemagglutinin / HA-specific B cell probe Protein Trimer (AVI & His Tag), Biotinylated (MALS-verified)
蛋白构建：A DNA sequence encoding the Influenza A H3N2 (A/District of Columbia/27/2023) Hemagglutinin / HA-specific B cell probe Protein Trimer (EPI2990337) (Met1-Val521) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 17
蛋白分子量：The recombinant Influenza A H3N2 (A/District of Columbia/27/2023) Hemagglutinin / HA-specific B cell probe Protein Trimer comprises 580 amino acids and has a predicted molecular mass of 64.31 kDa. As a result of glycosylation, the band migrates approximately 89.5 kDa in the SDS-PAGE reducing conditions. The molecular weight of this protein is around 251.3 kDa verified by SEC-MALS.
蛋白NP号：EPI2990337
蛋白氨基酸序列：Met1-Val521
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文献和实验Clonality - X Chromosome Inactivation Assay
from microdissected samples and "semi-purified" using a one-step proteinase K buffer strategy will sometimes produce "non-specific" PCR products in addition to the true alleles. Normal-cell DNA from archival specimens from control subjects serves as a good comparator
The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein
肝炎病毒包膜E2蛋白相同的亲和结合力3。另外，有阴离子转运载体AE1结构域的重组蛋白被标记且用Rho1D4系统纯化后表现出与红细胞来源的AE1蛋白有相同的硫酸外排速率。4） 利用Rho1D4纯化膜蛋白图解简述步骤1：结合：将Rho1D4标记的蛋白与载有Rho1D4抗体的琼脂糖一同孵育使得目标蛋白被固定在吸附材料上。 步骤2：清洗：将杂蛋白和其他裂解液成分洗去，留下与亲和基质结合的目标蛋白。 步骤3：洗脱：过量Rho1D4肽与基质竞争性结合，释放出目标蛋白在洗脱液中收集。 表
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