- ￥5170
- Sino Biological
- 北京
- 41056-V27H-B
- 2026年04月20日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Influenza A H3N2 (A/District of Columbia/27/2023) Hemagglutinin / HA-specific B cell probe Protein Trimer (AVI & His Tag), Biotinylated (MALS-verified)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg
蛋白名称：Influenza A H3N2 (A/District of Columbia/27/2023) Hemagglutinin / HA-specific B cell probe Protein Trimer (AVI & His Tag), Biotinylated (MALS-verified)
蛋白构建：A DNA sequence encoding the Influenza A H3N2 (A/District of Columbia/27/2023) Hemagglutinin / HA-specific B cell probe Protein Trimer (EPI2990337) (Met1-Val521) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-MALS.
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Gln 17
蛋白分子量：The recombinant Influenza A H3N2 (A/District of Columbia/27/2023) Hemagglutinin / HA-specific B cell probe Protein Trimer comprises 580 amino acids and has a predicted molecular mass of 64.31 kDa. As a result of glycosylation, the band migrates approximately 89.5 kDa in the SDS-PAGE reducing conditions. The molecular weight of this protein is around 251.3 kDa verified by SEC-MALS.
蛋白NP号：EPI2990337
蛋白氨基酸序列：Met1-Val521
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文献和实验Clonality - X Chromosome Inactivation Assay
from microdissected samples and "semi-purified" using a one-step proteinase K buffer strategy will sometimes produce "non-specific" PCR products in addition to the true alleles. Normal-cell DNA from archival specimens from control subjects serves as a good comparator
科研人必看：多肽、蛋白质、重组蛋白分不清？ 应用场景有哪些？定制如何选？
(Anti-FLAG 亲和凝胶纯化，温和洗脱，保持活性)、Strep 标签 (Strep-Tactin 亲和纯化，高纯度需求优选) 促溶标签：MBP、SUMO、GST 等，可显著提高难表达蛋白的可溶性 功能标签：Avi 标签 (常用于定点生物素化)、GFP (荧光标记，用于蛋白定位示踪) 无标签蛋白：如需天然构象或去除标签干扰，MCE 也支持无标签蛋白制备 『定制需求』 膜蛋白：拥有三大膜蛋白表达技术平台，包括去垢剂平台 (基于哺乳动物细胞表达系统)、纳米盘 (Nanodisc) 技术平台和病毒样
The multiple copies of the HA epitope present in the HAT tag can be detected by the mouse monoclonal antibodies 12CA5 (Boehringer) and 16B12 (MMS101R; BAbCO, Richmond, California) (see Table 1). On Western blots against yeast protein
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