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文献和实验1). Albumin or powdered milk is used to block the remaining sites which could result in false positives. Monoclonal antibodies will react with the antigen attached to the microtiter plate. The complex antigen-antibody is then reacted with enzyme conjugates
Polyclonal and Monoclonal Antibodies
or O.N at 4¡C. 4) SECONDARY ANTIBODY a) Wash unbound antibody 4 times with PBS + 0.1% Tween 20. b) Add 100 ul of enzyme linked antibody to all wells. Do the appropriate dilutions in the Elisa buffer. (ex: HRP is 2000X). c
Monoclonal Antibody Production Protocol
of 1/5 dilutions. Do dilutions in blocking solution. Leave 1 hr at room temp or O.N at 4°C. SECONDARY ANTIBODY Wash unbound antibody 4 times with PBS + 0.1% Tween 20. Add 100 ul of enzyme linked antibody to all wells. Do the appropriate dilutions in the Elisa
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