S. aureus RNA Polymerase Assay

Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit

into the pCRII vector. This plasmid was then used to generate RNA probes for use in a ribonuclease protection assay. Although much of the description of the ligation and transformation procedures is based on directions supplied by Invitrogen, some steps differ

mRNA Amplification with T7 RNA Polymerase

Materials MessageAmp II aRNA Amplication Kit (Cat #1751, Ambion) 100% Ethanol RNA samples (e.g. 1 µg RNA per sample) Procedure Reverse transcription Verify that EtOH (24 mL) has been added to the Wash Buffer. Turn

Use of Hybridization Probes in a Real-Time PCR Assay on the LightCycler for the Detection of Methicillin-Resistant Staphylococcus aureus

by the mecA gene in S. aureus . Because routine laboratory diagnostics may be time consuming and because species differentiation encounters a variety of difficulties, molecular techniques detecting both the mecA and a S. aureus -specific gene are used