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文献和实验Western Blotting with Horseradish Pe
. 3.Remove blot from the bag or tray and place it between two pieces of write-on acetate transparency film. Smooth over covered blot to remove air bubbles and excess substrate. EXPOSE TO X-RAY FILM OR ANY SENSITIVE SCREEN. AN INITIAL EXPOSURE OF 10-60
T-cell dependent antigen specific in situ staining (Pe)
26- counterstain with rabbit anti-goat-AP 1:200 27- wash thrice 28- develop AP (see protocol) HRP development protocol (AEC): Developing solution: For 50 ml developing solution add in order: Aminoethylcarbazole (20 mg tablets
Silver Acetate Autometallography (AMG)
and the detection of metallic gold and silver, and sulfides and selenides of mercury, silver and zinc. Solutions A and B should be freshly prepared for every run. Solution A: Dissolve 80 mg silver acetate (code 85140; Fluka, Buchs, Switzerland) in 40 mL
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