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Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
, add 0.1 ng/ml Hoechst 33258 in PBS for 15 minutes. 13) Rinse the samples with 3x 10 minute washes in PBS. 14) Analyze the samples expression of MHC and Cx43 by imaging the cells with a confocal microscope (See Figure 4 for example
and cathode buffers (see reciperecipes ) Electrophoresis apparatus: Protean II 16‐cm cell (Bio‐Rad) or SE 600 16‐cm unit (Hoefer) with clamps, glass plates, casting unit, and buffer chambers
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