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- TD-AB2009
- 2026年08月01日
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- 文献和实验
- 技术资料
- 形态：
Liquid
- 保存条件：
Store at 2-8°C under sterile conditions.
- 供应商：
武汉天德
- 抗体英文名：
Alkaline Phosphatase Goat Anti-Mouse IgG (H+L)
- 规格：
50ul
| Product | Alkaline Phosphatase Goat Anti-Mouse IgG (H+L) |
|---|---|
| Code Number | TD-AB2009 |
| Conjugate | Alkaline Phosphatase |
| Physical State | Liquid |
| Size | 20ul/50ul/100ul |
| Antibody Concentration | 0.6 mg/ml |
| Suggested Dilution Range | 1:5,000 - 1:50,000 for ELISA and Western blotting |
| Alkaline Phosphatase | Purified from calf intestine |
| Buffer | 0.01M Tris-HCl, 0.25M NaCl, pH 8.0 |
| Stabilizer | 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) |
| Preservative | 0.05% Sodium Azide |
| Storage | Store at 2-8°C under sterile conditions. |
| Purity | Affinity chromatography with Protein A/G |
| Background | Immunoglobulin G (IgG) is one of the most abundant proteins in serum with normal levels between 9.5-12.5 mg/ml in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants. |
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文献和实验Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Materials Anti-digoxigenin-AP conjugate (Roche #11093274910, 150U/200 uL, $168) Levamisole (Sigma #T1512, 2 g, $10) (blocks endogenous phosphatase activity) BCIP (Roche #11383221001, 3 mL, $49) NBT (Roche #11383213001, 3 mL, $48)
ALKALINE PHOSPHATASE (APAAP) TECHNIQUE
9. Wash in buffer. 3 minutes. 10. Incubate in rabbit anti-mouse immunoglobins. 30 minutes. 11. Jet wash in buffer. 12. Wash in buffer. 3 minutes. 13. Incubate in alkaline phosphatase-mouse anti-alkaline phosphatase 30 minutes. 14. Jet
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
dehydrogenases. Progr. Histochem. Cytochem . 4 :225. Berger, J., A.D. Howard, L. Gerber, B.R. Cullen, and S. Udenfriend. 1987. Expression of active, membrane-bound human placental alkaline phosphatase by transfected simian cells. Proc. Natl. Acad. Sci
