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- 详细信息
- 文献和实验
- 技术资料
- 形态:
Liquid
- 保存条件:
Store at 2-8°C under sterile conditions.
- 供应商:
武汉天德
- 抗体英文名:
Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L)
- 规格:
50ul
| Product | Alexa Fluor® 488 Goat Anti-Rabbit IgG (H+L) |
|---|---|
| Code Number | TD-AB2018 |
| Conjugate | Alexa Fluor® 488 |
| Physical State | Liquid |
| Size | 20ul/50ul/100ul |
| Antibody Concentration | 1.5 mg/ml |
| Suggested Dilution Range | 1:100 - 1:800 for most applications |
| Fluorophore | Alexa Fluor®488 carboxylic acidλmax=493 nm; Emax=519 nm |
| Fluorophore/Protein Absorbance Ratio | A493/A280 = 2.0 |
| Buffer | 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6 |
| Stabilizer | 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free) |
| Preservative | 0.05% Sodium Azide |
| Storage | Store at 2-8°C under sterile conditions. |
| Purity | Affinity chromatography with Protein A/G |
| Background | Immunoglobulin G (IgG) is one of the most abundant proteins in serum with normal levels between 9.5-12.5 mg/ml in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10¹¹ variants. |
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文献和实验FITC荧光二抗?――EarthOx新型DyLight 488绿色荧光
AffiniPure Goat Anti-Rabbit IgG(H+L) EarthOx E032220 ¥200/100μl ¥800/500μl 详情 DyLight 488 AffiniPure Rabbit Anti-Goat IgG(H+L) EarthOx E032230 ¥200/100μl
XXXX原代细胞干预培养与激光共聚焦蛋白细胞定位实验 实验试剂:PBS(磷酸盐缓冲液pH7.4)(实验室常规配置);非免疫山羊血清;Triton X-100(sigma);BSA抗体稀释液(实验室常规配置);A蛋白抗体(Santa cruz);B蛋白抗体(Abcam);Alexa Fluor® 488 donkey anti-goat IgG (H+L)(Invitrogen);Alexa Fluor® 555 Anti-Rabbit IgG (H+L), F(ab
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色(可选) 1. 将细胞重悬在 0.5 ml DNA 染色液中(例如 DRAQ5® #4084)。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗: 抗兔: Anti-Rabbit IgG(H+L), F(ab')2 Fragment(Alexa Fluor® 488 Conjugate)#4412 Anti-Rabbit IgG(H+L), F(ab
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