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文献和实验Human Mast Cell Proteases: Activity Assays Using Thiobenzyl Ester Substrates
Human mast cells contain proteases that are important functional components and serve as markers of mast cell activation or degranulation. Although tryptase is the best recognized mast cell protease, chymase and Cathepsin G
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
, adjust sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator
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