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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
BseSI (Bme1580I) (10 U/µL)
- 规格：
500 units
描述
| 5' | G | K | G | C | M ↓ | C | 3' | ||||
| 3' | C ↑ | M | C | G | K | G | 5' |
Thermo Scientific BseSI (Bme1580I) restriction enzyme recognizes GKGCM^C sites and cuts best at 55°C in G buffer (isoschizomers: BaeGI, BstSLI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
规格
| Compatible Buffer: | 10x Buffer G |
|---|---|
| Enzyme: | BseSI (Bme1580I) |
| Methylation Sensitivity: | Not CpG methylation-sensitive, Not dam methylation-sensitive, Not dcm methylation-sensitive |
| Optimal Reaction Temperature: | 55° C |
| Product Size: | 500 units |
| Sensitive to Heat Inactivation: | Yes |
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文献和实验RUVKUN Antibody Staining Protocol revised 10/29/90
RUVKUN Antibody Staining Protocol revised 10/29/90 Fixation: 1. C. elegans N2 grown in liquid or plates. Harvest worms. Wash for 2 hours at room temp. in 1X M9 or PBS. 2. Fix in 5 ml of FRESH THAT DAY 1% paraformaldehyde:
) protein, load only 0.0125 OD ml. For Drosophila tissue culture cells, suspend 3X106 cells per 90 µl cracking buffer, and load about 20 µl per lane (0.8 mm thick gel with the BRL gel box and 20 well comb). 10. Running. Use a constant Amps power supply
Protein Expression and Purification of MBP-T antigen (131-259)
elutes as a single sharp peak.Collect the fraction and freeze the protein at -80 ℃ until further use. Buffer A 50 mM HEPES,0.3 M NaCl,1 mM BME,10 mM Imidazole,1 tablet PI/L,pH 7.5 Buffer B 50 mM HEPES,0.3 M NaCl,1 mM BME,300 mM Imidazole,1 tablet PI
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