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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Human recombinant protein fragment of Human INHBE (NP_113667) produced in Ecoli.
- 保存条件:
Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
- 克隆性:
Monoclonal
- 适应物种:
Human, Mouse
- 保质期:
Stable for 12 months from date of receipt.
- 目录编号:
CF815947
- 级别:
科研级
- 库存:
2周
- 供应商:
OriGene
- 宿主:
Mouse
- 应用范围:
WB
- 靶点:
INHBE
- 抗体英文名:
Carrier-free (BSA/glycerol-free)INHBE mouse monoclonal antibody,clone OTI24A7
- 抗体名:
Carrier-free (BSA/glycerol-free)INHBE mouse monoclonal antibody,clone OTI24A7
- 规格:
100 ug
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文献和实验Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
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