- ￥3990
- OriGene
- 美国
- CF815852
- 2026年04月01日
- WB
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human recombinant protein fragment of Human INHBE (NP_113667) produced in Ecoli.
- 保存条件：
Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
- 克隆性：
Monoclonal
- 适应物种：
Human
- 保质期：
Stable for 12 months from date of receipt.
- 目录编号：
CF815852
- 级别：
科研级
- 库存：
2周
- 供应商：
OriGene
- 宿主：
Mouse
- 应用范围：
WB
- 靶点：
INHBE
- 抗体英文名：
Carrier-free (BSA/glycerol-free)INHBE mouse monoclonal antibody,clone OTI14B5
- 抗体名：
Carrier-free (BSA/glycerol-free)INHBE mouse monoclonal antibody,clone OTI14B5
- 规格：
100 ug
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文献和实验Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
monoclonal antibody production fusion
^6 cells/ml (IMDM + 10% FCS/FBS). Just before the fusion wash 3 times in IMDM (serum free!), 1200 rpm, 5min, 37ѓC. Count cells. sacrifice the mouse and dissect the spleen. tease the spleen in ice-cold IMDM (serum free!); pass
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