cfRNA can be eluted into 15 µl (to maximize total yield) or as little as 6 µl (for highly concentrated eluate) of DNase/RNase-Free Water.
Processing Time
Typically requires 30 minutes hands-on time to process 10 samples. Sample digestion requires 2 hours.
Purity
High quality cell-free RNA (cfRNA) is ready for all sensitive downstream applications such as RT-qPCR and Next-Generation Sequencing.
Required Equipment
Microcentrifuge, vortex, water bath or heating block, vacuum, and vacuum manifold.
Sample Type
Up to 3 ml of plasma, serum, cerebrospinal fluids, amniotic fluids, urine, or other biological fluids.
Size
RNA ≥ 17 nt.
Yield
Yields are sample dependent and may vary. Typical recovery ranges from 1 – 100 ng/ml of human plasma or serum. Kit is optimized for recovery of total cfRNA, including small RNA and microRNA.