鼻拭子，鼻咽拭子，唾液RNA提取试剂盒

鼻拭子，鼻咽拭子，唾液RNA提取试剂盒
EpiQuik Viral RNA Isolation Fast Kit
唾液RNA提取试剂盒
鼻拭子RNA提取试剂盒
鼻咽拭子RNA提取试剂盒

Input Type:	Nasal Swab, Nasopharyngeal Swab, Saliva
Research Area:	Epidemiology
Target Application:	Sample Isolation
Vessel Format:	Columns/Tubes
100% Guarantee:	6 months

The EpiQuik™ Viral RNA Isolation Fast Kit is a complete set of optimized buffers and reagents suitable for quick preparation of viral RNA from cell-free liquid specimens (excluding plasma and serum), specifically from saliva and nasal or nasopharyngeal swabs. The specialized buffering system allows RNA to bind to the glass fiber matrix of the spin column while contaminants pass through the column. Impurities are efficiently washed away, and pure RNA is eluted. The RNA purified with the kit can be used for a variety of routine applications, specifically for RT-PCR. This kit has the following advantages and features:

• Fast procedure delivers high-quality total RNA in total 10 minutes
• Ready-to-use RNA for high performance in downstream applications
• Consistent RNA yield from a small amount of starting material

Total RNA was isolated from nasal swab samples with the EpiQuik™ Viral RNA Isolation Fast Kit and used for RT-PCR analysis. A: Nasopharyngeal swab samples were spiked with SARS-CoV-2-N positive control; B: Nasopharyngeal swab samples were not spiked with SARS-CoV-2-N positive control. PCR was processed with use of primers and probes against SARS-CoV-2 N1/N2 and human RNase P gene (internal control).

Principle & Procedure
The kit contains all the reagents required for successfully performing RNA isolation directly from cell-free samples (excluding plasma and serum). After lysis, binding, and wash, RNA is easily recovered in quantities of up to 5 µg using specially designed columns. Total RNA is then ready to be used for a variety of downstream applications.

Starting Materials
The amount of starting materials can be up to 400 µl of liquid volume, with the best volume of 200 µl. A total of 50 standard extractions (using 200 µl of sample) can be performed with this kit.

Competitor Kit Comparison

Kits	EpiQuik Viral RNA Isolation Fast Kit	Competitor Q Kit	Competitor Z Kit	Competitor O Kit
Sample Types	Cell-free biological fluids and liquid specimens (excluding plasma and serum)
Run Time	10 minutes	20-40 minutes	10 minutes	15-20 minutes
Yield	>95% recovery	>90% recovery	Unspecified in documentation	Unspecified in documentation
Price Per Preparation	$	$$$	$$	$$
Processing	Manual	Manual; Automation (requires vendor-specific equipment)	Manual	Manual
Sample Input Volume	400 µl	140 µl	400 µl	150 µl