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- LV900-R
- 2025年11月12日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
qPCR Lentivirus Complete Titration Kit (MasterMix-R)
- 规格：
100 Reactions
provides a fast and simple method for titrating lentivirus. This kit employs a quick RNA extraction step and determines viral RNA using qRT-PCR. The whole assay could be completed in only 2 hours. This complete kit differs from the standard qPCR Lentivirus Titration Kit (Cat. No. LV900) in that it contains the Lentivirus 2X qPCR MasterMix necessary for the real-time PCR reaction. Please refer to our qPCR MasterMix Selection Guide for selecting the appropriate qPCR formulation applicable to your particular instrument model. The titer primers supplied in this kit are designed for all HIV-1 based vectors detection. With the help of the on-line titer calculator, titration of any lentiviral preparation could be easier than ever before.
- no RNA purification -- saves time and eliminates inaccuracy
- ready-to-use reagent mix -- reduces variability
- qRT-PCR in one step -- more sensitive and accurate than other methods
- no NTC amplification -- our titration kit is the only one on the market that completely eliminates NTC signal due to our unique primer design. Similar kits from other companies all give NTC amplification, which compromise accuracy for viral titration
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文献和实验可以获得真核生物 mRNA 全长；当 RNA 没有 polyA 尾（原核 mRNA 或 rRNA）或断裂时，就需要考虑使用随机引物进行 RNA 的反转录，随机引物可以很均一的逆转出 RNA 上的信息，但逆转出的 cDNAs 长度都较短。此时，对于扩增真核生物的 total RNA 来说，这时随机引物和 Oligo-dT 引物的混合物能给出一个令人满意的结果。 诺唯赞的 HiScript® III 1st Strand cDNA Synthesis Kit (+gDNA wiper)（R312）或可助
polymerase. Use this as a starting point when using previously untested primers and templates. Optimal concentrations may vary from 0.5 to 6 mM and are determined by titration with MgCl 2. Because of this variation, 10X stock solutions frequently
RAMPAGE: Promoter Activity Profiling by Paired‐End Sequencing of 5′‐Complete cDNAs
and mapping of promoters for analysis of gene expression (RAMPAGE) is a method that harnesses highly specific sequencing of 5??complete complementary DNAs to identify transcription start sites (TSSs) genome?wide. Although TSS mapping has historically relied
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