Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.
Highlights
• Convenient, ready-to-use mix • Thermostable—half life is more than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)
Applications
• Routine PCR amplification of DNA fragments up to 5 kb
• High throughput PCR
• DNA labeling
Note
• The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.
规格
Polymerase:
Taq DNA Polymerase
Hot Start:
No
Fidelity (vs. Taq):
1 X
GC-Rich PCR Performance:
Low
Reaction Format:
SuperMix or Master Mix
Product Overhang:
3'-A
Reaction Speed:
Standard
Product Size:
1,000 reactions
内容及储存
Contains:
• 20 x 1.25 mL PCR Master Mix (2X), which includes:
— Taq DNA polymerase (0.05 U/µL),
— reaction buffer,
— 4 mM MgCl2, and
— 0.4 mM of each dNTP
• 20 x 1.25 mL Nuclease-free water