RP105 (CD180) mouse monoclonal

Rapid Isotyping of Mouse Monoclonal Antibodies

for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance

Purification of Endotoxin Free mAbs

. If you have larger volumes, or your antibody is present at low concentrations/poor yields, we find it useful to concentrate the supernatants by circulating them through the same type of hollow fibre dialysis cartridges we use for cell growth, allowing the slight

Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas

are very stable - even in long term large scale cultures (bioreactors). b) High levels of monoclonal antibody secretion in culture - often 100 micrograms per ml of ordinary tissue culture, without recloning, and up to 5 milligrams per ml in hollow fibre