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- 文献和实验
- 技术资料
- 形态:
Lyophilized or Liquid
- 保存条件:
-20°C
- 克隆性:
Polyclonal
- 宿主:
Rabbit
- 应用范围:
ICC=1:50-200 IF=1:50-200
- 浓度:
1mg/ml
- 抗体名:
Cy5.5标记的胞质紧密粘连蛋白1/闭锁小带蛋白1抗体
- 规格:
100ul
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文献和实验derived monoclonal antibodies, XH1 and XH2, to compete with the well-characterized ZO-1 anti-YFP1 antibody for binding to the target antigen YFP1; hypothetical antibody PC10 is used as a control. First, 96-well plates are coated with the YFP1 antigen
Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend
----------------------- Screening for Protein-Protein Interactions. Protein G was from Pierce (Rockford, IL) and BODIPY-FL-Goat-anti-Mouse IgG was from Molecular Probes (Eugene, OR). IKBalpha and p50 were kindly provided by T. Maniatis (Harvard University, Cambridge, MA
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