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文献和实验SDS-PAGE Western Blotting Protocols
glass plate can be treated with Rain-X to facilitate later removal of the gel. 2. Slide into holder without tightening the screws. 3. Place on pouring stand, making sure that the glass plates are pushed all the way to the bottom before tightening
ml bottles) for 15 min. 4. Pull off serum by aspiration (peelet is soft and will not stick to bottle). 5. Filter serum successively through 1.2 µm, .85µm and .65 µm filters using 142 mm filter holder. 6. Adjust pH to 7.0 using 1N NaOH. 7. Heat
at 750 ng/ul for RLGS analysis in this plant. Ascertain the size of obtained DNA fragments are larger than lamda DNA using 0.5 % agarose gel electrophoresis (100 - 200 kb recommended). B. Preparation of gel holder Cut teflon-tubing (NICHIAS AWG
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