ANTI-PCNA (PROLIFERATING CELL

Immunohistochemical Detection of Cells in the Division Cycle Using Antibodies to Proliferating Cell Nuclear Antigen (PCNA)

of the growth fraction. Proliferating cell nuclear antigen (PCNA) is an endogenous nuclear protein that functions as an accessory protein to DNA polymerase (pol) δ (1 ). Since PCNA is present in all cycling cells, the entire proportion of dividing cells present

Analysis of Proliferating Cell Nuclear Antigen (PCNA) Associated With DNA Excision Repair Sites in Mammalian Cells

Proliferating cell nuclear antigen (PCNA) is a homotrimeric protein adopting a ring structure that may encircle DNA. In this form, PCNA functions as a sliding platform to which different types of catalytic and regulatory proteins are tethered

IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Vector Red Detection

in a humid chamber for 1 hour, RT. Blot off antibody, wash 2 times 5 min. each in 1x PBS. Prepare the working dilution of the secondary antibody (Horse anti-mouse IgG-Vector Catalog No. BA2001) in 1.0% BSA/PBS, 2.0% normal horse serum. Prepare