- ￥100 - 9999
- Bechman COULTER
- A64336
- 2026年03月21日
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文献和实验UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
. 9. Open exhaust heater condensate valve soon after sterilization cycle is complete (during cooldown cycle is OK) . 10. Adjust air regulator on the front of fermentor to 35 PSIG. Adjust air flow valve to desired value. Usually about 12-15
, and aliquot 3 ml into sterile glass tubes, and place in a 50℃ temp.block. To plate a transformation mix: just before use, add 40 µl 24 mg/ml IPTG, 40 µl 20 mg/ml Xgal in DMF, 10 µl 20 mg/ml amp (or carb) (store all these stocks separately at -20℃; you can mix
temp. during the transformation. Melt some L soft agar in the microwave, and aliquot 3 ml into sterile glass tubes, and place in a 50℃ temp.block. To plate a transformation mix: just before use, add 40 µl 24 mg/ml IPTG, 40 µl 20 mg/ml Xgal in DMF, 10 µl
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