- ￥2800
- 戴格生物
- 中国
- PKT1092C-PBS
- 2026年03月20日
- ELISA
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
IgG
- 保存条件：
Store at -20°C. Recommended to aliquot into single-use vials. Supplied in 1X PBS (pH 7.4). BSA and Azide Free. Stable for 12 months from date of receipt.
- 克隆性：
Monoclonal
- 标记物：
Un-conjugated
- 适应物种：
Human
- 供应商：
杭州戴格生物技术有限公司
- 宿主：
Rabbit
- 应用范围：
ELISA
- 浓度：
2 mg/ml
- 靶点：
FSTL1/FRP
- 抗体名：
FSTL1/FRP抗体对
- 规格：
100μg
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文献和实验. For primary antibody use anti-GST at 1:1000 and anti-actin at 1:1000. For secondary use HRP conjugated IgG at 1:10,000. Actin Capture Assay Reagants Binding Buffer: 1X PBS pH 7.2 (Lane Recipe) + 10% Glycerol + 1mMMgCl2 + 0.2mM ATP + 0.1% BSA
Actin Capture Assay David Amberg Dialyze purified GST fusion proteins and actin into PBS + 1mM MgCl2 . Mix 5ug actin into 50ul total volume binding buffer. Mix 5ug GST-fusion protein into total volume 50ul binding
Immuno-Laser Capture Microdissection
with the primary and secondary antibodies and the tertiary reagent for 90 to 120 seconds each, rinsing briefly with 1 x PBS between each step. Develop the color with diaminobenzidine (DAB) for 3 to 5 minutes and counterstain with hematoxylin for 15 to 30
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