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文献和实验Coupling Peptides To Resin For Affinity Purification
volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add
Immunofluorescence on ultrathin resin sections
. References: Immunofluorescence on ultrathin resin sections: Albrecht U, Seulberger H, Schwarz H, and Risau W. (1990) Brain Res.535, 49-61. Schwarz H, Hohenberg H, and Humbel BM. (1993) Immuno-Gold
Appendix H: Radioactive tracers
TABLE H.1 Radioactive Sources and Emission Types MEASUREMENT OF DOSE When alpha or beta particles, or gamma radiation pass through matter, they form ions. They accomplish this by knocking electrons from the orbits
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