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文献和实验Metaphase chromosome preparation Materials: RPMI 1640 medium fetal calf serum (FCS), 20% Colcemid (e.g. Boehringer Mannheim cell biology reagents, Best.-Nr. 295892) cell cuture flask Phythemaglutinin, PHA-L (Seromed, M
Neuropeptide Localization in Nonmammalian Vertebrates
the molecular expression of proteins in tissues and cells of different classes of vertebrates and has been fully exploited in the study of the mammalian brain. The present chapter provides a detailed description of the protocols routinely
Neuropeptide RNA Localization in Tissue Sections
In situ hybridization has become a routine technique to provide insights into RNA localization. However, different protocols exist for multiple purposes, and it is, therefore, important to clearly define specific needs
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