pCMV-Slf2-3×FLAG-Neo plasmid

pCMV-Slf2-3×FLAG-Neo plasmid

PVT112029

pCMV-Slf2-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Slf2(mouse)-3×FLAG-Neo
Alias: NM_001081225.3;3632432H19; Fam178a
Gene lenght: 3489
Host: Mammalian cells
Use(s): Gene expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Slf2-3×FLAG-Neo plasmid Description

pCMV-Slf2-3×FLAG-Neo It is a plasmid suitable for gene expression in mammalian cells.

pCMV-Slf2-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgac aaggcgctgc atgcccgcta 781 ggccaggttt cccctcatcc ccagccccgg ggtcgtcgcc cccgcgctgc catctgagac 841 ccggtagtac cgcccctgct gcagcgggaa aaagaacaga gagtcctggg gacaggaaac 901 agtcaatcat agatttcttc aagccagctg caaaacaaga caaacatatg ttggattcgc 961 ctcaaaaatc aaacatcaaa tacagaggaa atggattatc catcactggg acagagcaat 1021 ttgaaaggaa attatcttca ccaaaaaaac ttaaacctaa gaggatgtca tcagaagaaa 1081 gtcctatttt agaggctttc atgaaaggtg ggaaggagca ccacaaagat cgtggtgtcc 1141 atgagtcacg gcggccttgt atgtctctaa gcaaatattt acccaaagga gctggcatct 1201 atgctccttc ctcttatcga ttgcccaaag agatcaaggc acagaagaaa aaacaccagt 1261 ctcctgagag aaggaagtca ctgttcattc atgaaagtaa cagggagaag aatgacagag 1321 atcgaggcaa gaacagtgaa gactccagaa aacaggccac agccacagaa ggtgacatct 1381 tcaagcatag ctccagaagt attagtagca gaagcagcct gtccaggcac catccaggag 1441 aaagcacact gggggctagg ttccagctgt cattagcttc ttactgccga gagcgagaac 1501 agaagaagtt gagaaaggaa caaatggagc agagaatcaa ctcggaaaat tctttctcag 1561 aagcaagcaa cctttcctta aagtcgtctg gtgtagggaa aaactgtaag ccgaggcatg 1621 agcatagcaa gcacactgag gctgtacctg ggaagagtaa tctctcaact ctggaaaatg 1681 gacatctctc aagaaaaaga tcatcttcag actcgtggga actttcaggc tctaaacaga 1741 ataaattctc cgacaaaaga aagaggaact ctgtggactc agacctgaaa agcacaaaag 1801 aacctatcat accaaaagca agagagtcct ttctagagaa gcgcccagac acatcccatc 1861 agagagaaaa gtttatcaga cacattgcac tgaagactcc tggtggtgtt ctgcgcttgg 1921 aagacatagc caaggagcct gaggacgaga cagatcgctc ttctgcagac tcggcacctt 1981 caaatgctgg ccaccattct tccaggaata gtgaccaagt ccactcagca agtaccaaag 2041 agactaaaat acagaaaccc cacttacctt tacctcagga aaaatctaca attaaaagag 2101 ctagcaacct tcagaaaaac aaaccggctg gctctgtgac atcaaaagag acaaagctac 2161 ctttactttc ccatgttcca agtgctgttt cctctcgagt accactaaat gctaaaaatt 2221 gcactcttcc agttcctaaa aaagataaag agcgttcctc ctcaaaagaa cgttctgggc 2281 attctacaga atcctccaaa cacaaggaac acagagcaaa gactattaag gctgtttcta 2341 atgaatcgtc agggaaaaat tctgggggat ctttgcattc agagtatgcc cctccaacag 2401 catctccccc agctgccttg gaagtcgtgc cgagtgtccc aagccctgca gcaccttcag 2461 ataaagagag ttcaggaaat tccaatgcag gtagcaatgc actgaaaagg aaattcaggg 2521 gtgattttga tagtgatgaa gaaagtttag gttacacctt agagagtgat gaggaagagg 2581 aaacactaaa atcactggaa gaaataatgg ctctgaactt cagtcggact cctacaactt 2641 caggaaagcc tcctgcggtt tccaaggggc ttagatccca gtcgtcagac tacatggaat 2701 atgctcagag cggaacttac acaaatacct tagagcgact agtaaaggag atggaagaca 2761 cgcagaggct agatgaacta cagaagaagc tacaggaaga cataagacaa ggccgtggca 2821 ttaaatctcc tctcagaact ggcgatcagg atagtacaga tgatggtgat ggcctcttgg 2881 aagaacacag ggaatttcta aagaaatttt cagttacagt tgatgctatt cctgatcatc 2941 atccaggtga agaaatattt aatttcctca attctggaaa aattttcaat cagtatacct 3001 tagatttaag agactctggt tttattggag aaagtgctgt ggaaaagctt attctcaaat 3061 caggaaaaac agatcagatt tttttgacaa cacaaggctt ccttaccact gcatatcact 3121 acgtccaatg tcctgtacct gtgttaaagt ggctgtttcg gatgatgtcg gttcacacag 3181 attgtattgt gtcggtgcag attttaagta cattgatgga aataaccatt agaaatgata 3241 ctttcagtga ctctccagtt tggccctgga tcccatcttt gtctgatata gcagctgtat 3301 tcttcaacat gggcgttggt tttggatctt tgtttcctct ggagactctt cagcctgact 3361 ttaatgaaga aaatctaatt tctgaaacac agaaaacact ggggggaaaa gaaagtgaag 3421 attcacctta tagtccagta ttttcagctc ttcctgaaac caacatttta aatgtggtta 3481 agttcctagg tctgtgcaca tctatacatc cagaaggtta ccaagatgga gaattaatgc 3541 tgctgatttt aatgctgttt aaaatgagtt tggaaaaaga gcttaaacag attccattag 3601 tagactttca gagcctcctg ataaatctca tgaaaaacat cagagattgg aacacaaagg 3661 ttcatgaact ctgtctgggc ataaatgaac tctccagtca tccccataac cttctttggt 3721 tggtacagct agtccctaac tggacatctc gtggaaggca gctgaggcag tgcctcagtc 3781 tggtgatgat gtcaaagctt ttggatgaaa agcatgaaga tattcctaat gccaataacc 3841 ttcagatctc ggtcctgcat cgatatcttg tgcagatgaa gccttctgat ttgttgaaga 3901 aaatggtctt gaagaaaagg gccgaacagc caaatgaaac tattgatgac agtcttcatt 3961 tagaacttga aaagcaggca tattacctga cctacatcct tcttcattta gttggggaag 4021 ttagttgctc tcattctctt tcttctggac aacggaaaca cttcgtgctg ctttgtgggg 4081 ctttggaaaa gcatgttaag tgtgatatta gggaagatgc aagacttttc tacagaacta 4141 aagtgaaaga cttggttgcc agaatacatg gaaaatggca ggaaataatt cagaactgtc 4201 ggcctactca ggggcagctt catgacttct gggtgccaga ttctGGATCC GACTACAAAG 4261 ACCATGACGG TGATTATAAA GATCATGACA TCGACTACAA GGATGACGAT GACAAGTAGT 4321 GAGGGCCCGA TATCTCGACA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG 4381 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA 4441 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT 4501 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT 4561 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC 4621 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG 4681 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAGCTGAC 4741 GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG 4801 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT 4861 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC 4921 CTCCCCGCCT GGAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA 4981 ACCCGCGCTA TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGGTGTTG GGTCGTTTGT 5041 TCATAAACGC GGGGTTCGGT CCCAGGGCTG GCACTCTGTC GATACCCCAC CGAGACCCCA 5101 TTGGGGCCAA TACGCCCGCG TTTCTTCCTT TTCCCCACCC CACCCCCCAA GTTCGGGTGA 5161 AGGCCCAGGG CTCGCAGCCA ACGTCGGGGC GGCAGGCCCT GCCATAGCAG ATCTGCGCAG 5221 CTGGGGCTCT AGGGGGTATC CCCACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT 5281 GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC 5341 TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG 5401 CATCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA 5461 GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT 5521 GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT 5581 CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGGGGATT TCGGCCTATT GGTTAAAAAA 5641 TGAGCTGATT TAACAAAAAT TTAACGCGAA TTAATTCTGT GGAATGTGTG TCAGTTAGGG 5701 TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG 5761 TCAGCAACCA GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG 5821 CATCTCAATT AGTCAGCAAC CATAGTCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT 5881 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG 5941 GCCGAGGCCG CCTCTGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC 6001 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG 6061 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC 6121 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT 6181 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG 6241 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG 6301 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 6361 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA 6421 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC 6481 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC 6541 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG 6601 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG 6661 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT 6721 GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC 6781 GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC 6841 ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGCGAAAT 6901 GACCGACCAA GCGACGCCCA ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA 6961 TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG 7021 GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA 7081 CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG 7141 TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG 7201 CTAGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 7261 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 7321 GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 7381 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 7441 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT 7501 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 7561 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 7621 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 7681 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 7741 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 7801 AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 7861 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 7921 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 7981 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 8041 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 8101 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 8161 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 8221 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 8281 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 8341 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG 8401 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT 8461 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC 8521 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC 8581 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG 8641 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC 8701 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG 8761 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC 8821 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT 8881 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC 8941 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT 9001 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC 9061 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC 9121 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA 9181 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT 9241 CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG 9301 ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG 9361 AAAAGTGCCA CCTGACGTCG ACGGATCGGG AGATCTCCCG ATCCCCTATG GTCGACTCTC 9421 AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATC TGCTCCCTGC TTGTGTGTTG 9481 GAGGTCGCTG AGTAGTGCGC GAGCAAAATT TAAGCTACAA CAAGGCAAGG CTTGACCGAC 9541 AATTGCATGA AGAATCTGCT TAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.