pESC-GAL1-HMOX1-FLAG-GAL10-POR

pESC-GAL1-HMOX1-FLAG-GAL10-POR-Myc-URA3 plasmid

PVT111861

pESC-GAL1-HMOX1-FLAG-GAL10-POR-Myc-URA3 plasmid Information

Plasmid name: pESC-GAL1-HMOX1(human)-FLAG-GAL10-POR(human)-Myc-URA3
Alias: NM_002133.3/NM_001395413.1;bK286B10; HMOX1D; HO-1; HSP32；CPR; CYPOR; P450R
Gene lenght: 2895
Host: Yeast
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: URA3
Fluorescent labeling:
Promoter: GAL1,10
Replicon: 2U
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GAL1-F1
Reverse primer:
Copy number: High
Induction:

pESC-GAL1-HMOX1-FLAG-GAL10-POR-Myc-URA3 plasmid Description

pESC-GAL1-HMOX1-FLAG-GAL10-POR-Myc-URA3 is a plasmid suitable for gene expression in yeast.

pESC-GAL1-HMOX1-FLAG-GAL10-POR-Myc-URA3 plasmid Sequence

1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATACCAC AGCTTTTCAA TTCAATTCAT CATTTTTTTT TTATTCTTTT TTTTGATTTC 241 GGTTTCTTTG AAATTTTTTT GATTCGGTAA TCTCCGAACA GAAGGAAGAA CGAAGGAAGG 301 AGCACAGACT TAGATTGGTA TATATACGCA TATGTAGTGT TGAAGAAACA TGAAATTGCC 361 CAGTATTCTT AACCCAACTG CACAGAACAA AAACCTGCAG GAAACGAAGA TAAATCATGT 421 CGAAAGCTAC ATATAAGGAA CGTGCTGCTA CTCATCCTAG TCCTGTTGCT GCCAAGCTAT 481 TTAATATCAT GCACGAAAAG CAAACAAACT TGTGTGCTTC ATTGGATGTT CGTACCACCA 541 AGGAATTACT GGAGTTAGTT GAAGCATTAG GTCCCAAAAT TTGTTTACTA AAAACACATG 601 TGGATATCTT GACTGATTTT TCCATGGAGG GCACAGTTAA GCCGCTAAAG GCATTATCCG 661 CCAAGTACAA TTTTTTACTC TTCGAAGACA GAAAATTTGC TGACATTGGT AATACAGTCA 721 AATTGCAGTA CTCTGCGGGT GTATACAGAA TAGCAGAATG GGCAGACATT ACGAATGCAC 781 ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG GTTTGAAGCA GGCGGCAGAA GAAGTAACAA 841 AGGAACCTAG AGGCCTTTTG ATGTTAGCAG AATTGTCATG CAAGGGCTCC CTATCTACTG 901 GAGAATATAC TAAGGGTACT GTTGACATTG CGAAGAGCGA CAAAGATTTT GTTATCGGCT 961 TTATTGCTCA AAGAGACATG GGTGGAAGAG ATGAAGGTTA CGATTGGTTG ATTATGACAC 1021 CCGGTGTGGG TTTAGATGAC AAGGGAGACG CATTGGGTCA ACAGTATAGA ACCGTGGATG 1081 ATGTGGTCTC TACAGGATCT GACATTATTA TTGTTGGAAG AGGACTATTT GCAAAGGGAA 1141 GGGATGCTAA GGTAGAGGGT GAACGTTACA GAAAAGCAGG CTGGGAAGCA TATTTGAGAA 1201 GATGCGGCCA GCAAAACTAA AAAACTGTAT TATAAGTAAA TGCATGTATA CTAAACTCAC 1261 AAATTAGAGC TTCAATTTAA TTATATCAGT TATTACCCTA TGCGGTGTGA AATACCGCAC 1321 AGATGCGTAA GGAGAAAATA CCGCATCAGG AAATTGTAAA CGTTAATATT TTGTTAAAAT 1381 TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA 1441 TCCCTTATAA ATCAAAAGAA TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA 1501 AGAGTCCACT ATTAAAGAAC GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG 1561 GCGATGGCCC ACTACGTGAA CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA 1621 AAGCACTAAA TCGGAACCCT AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG 1681 CGAACGTGGC GAGAAAGGAA GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA 1741 GTGTAGCGGT CACGCTGCGC GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG 1801 GCGCGTCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC 1861 TTCGCTATTA CGCCAGCTGA ATTGGAGCGA CCTCATGCTA TACCTGAGAA AGCAACCTGA 1921 CCTACAGGAA AGAGTTACTC AAGAATAAGA ATTTTCGTTT TAAAACCTAA GAGTCACTTT 1981 AAAATTTGTA TACACTTATT TTTTTTATAA CTTATTTAAT AATAAAAATC ATAAATCATA 2041 AGAAATTCGC TTATTTAGAA GTGTCAACAA CGTATCTACC AACGATTTGA CCCTTTTCCA 2101 TCTTTTCGTA AATTTCTGGC AAGGTAGACA AGCCGACAAC CTTGATTGGA GACTTGACCA 2161 AACCTCTGGC GAAGAATTGT TAATTAAGAG CTCAGATCTT ATCGTCGTCA TCCTTGTAAT 2221 CCATCGATAC TAGTTCcatg gcataaagcc ctacagcaac tgtcgccacc agaaagctga 2281 gtgtaaggac ccatcggaga agcggagcct gggagcgggt gttgagtggg ggcttccctc 2341 tgggagtctc cacgggggca gaatcttgca ctttgttgct ggcccgctgg cgaagccctg 2401 gtgcccgtga ggggctctgg tccttggtgt catgggtcag cagctcctgc aactcctcaa 2461 agagctggat gttgagcagg aacgcagtct tggcctcttc tatcaccctc tgcctgactg 2521 cgggagtcat ctccagggag ttcatgcggg agcggtagag ctgcttgaac ttggtggcac 2581 tggcaatgtt ggggaaggtg aagaaggcca ggccctcgcc agagctgggc aggtccaggg 2641 ctttctgggc aatctttttg agcacctggc ccccagacag gtcacccagg tagcgggtgt 2701 aggcgtgggc caccagcagc tcgggctctg tgcgccccac ctcgtggagc cgcttcacat 2761 agcgctgcat ggctggtgtg taggggatga cctcctgcca gcggggcccg taccagaagg 2821 ccaggtcctg ctccagggca gccttgcggt gcagctcttc tgggaagtag acaggggcga 2881 agactgggct ctccttgttg cgctcaatct cctcctccag ggccacatag atgtggtaca 2941 gggaggccat caccagcttg aagccgtctc gggtcacctg gcccttctga aagttcctca 3001 tgaactcagc attctctgcc tgggtgtgca cctccttggt ggcctccttc agggcctctg 3061 acaaatcctg gggcatgctg tcgggttgcg gacgctccat GAATTCGAAT TTTCAAAAAT 3121 TCTTACTTTT TTTTTGGATG GACGCAAAGA AGTTTAATAA TCATATTACA TGGCATTACC 3181 ACCATATACA TATCCATATA CATATCCATA TCTAATCTTA CTTATATGTT GTGGAAATGT 3241 AAAGAGCCCC ATTATCTTAG CCTAAAAAAA CCTTCTCTTT GGAACTTTCA GTAATACGCT 3301 TAACTGCTCA TTGCTATATT GAAGTACGGA TTAGAAGCCG CCGAGCGGGT GACAGCCCTC 3361 CGAAGGAAGA CTCTCCTCCG TGCGTCCTCG TCTTCACCGG TCGCGTTCCT GAAACGCAGA 3421 TGTGCCTCGC GCCGCACTGC TCCGAACAAT AAAGATTCTA CAATACTAGC TTTTATGGTT 3481 ATGAAGAGGA AAAATTGGCA GTAACCTGGC CCCACAAACC TTCAAATGAA CGAATCAAAT 3541 TAACAACCAT AGGATGATAA TGCGATTAGT TTTTTAGCCT TATTTCTGGG GTAATTAATC 3601 AGCGAAGCGA TGATTTTTGA TCTATTAACA GATATATAAA TGCAAAAACT GCATAACCAC 3661 TTTAACTAAT ACTTTCAACA TTTTCGGTTT GTATTACTTC TTATTCAAAT GTAATAAAAG 3721 TATCAACAAA AAATTGTTAA TATACCTCTA TACTTTAACG TCAAGGAGAA AAAACCCCGG 3781 ATCCatggga gactcccacg tggacaccag ctccaccgtg tccgaggcgg tggccgaaga 3841 agtatctctt ttcagcatga cggacatgat tctgttttcg ctcatcgtgg gtctcctaac 3901 ctactggttc ctcttcagaa agaaaaaaga agaagtcccc gagttcacca aaattcagac 3961 attgacctcc tctgtcagag agagcagctt tgtggaaaag atgaagaaaa cggggaggaa 4021 catcatcgtg ttctacggct cccagacggg gactgcagag gagtttgcca accgcctgtc 4081 caaggacgcc caccgctacg ggatgcgagg catgtcagcg gaccctgagg agtatgacct 4141 ggccgacctg agcagcctgc cagagatcga caacgccctg gtggttttct gcatggccac 4201 ctacggtgag ggagacccca ccgacaatgc ccaggacttc tacgactggc tgcaggagac 4261 agacgtggat ctctctgggg tcaagttcgc ggtgtttggt cttgggaaca agacctacga 4321 gcacttcaat gccatgggca agtacgtgga caagcggctg gagcagctcg gcgcccagcg 4381 catctttgag ctggggttgg gcgacgacga tgggaacttg gaggaggact tcatcacctg 4441 gcgagagcag ttctggccgg ccgtgtgtga acactttggg gtggaagcca ctggcgagga 4501 gtccagcatt cgccagtacg agcttgtggt ccacaccgac atagatgcgg ccaaggtgta 4561 catgggggag atgggccggc tgaagagcta cgagaaccag aagcccccct ttgatgccaa 4621 gaatccgttc ctggctgcag tcaccaccaa ccggaagctg aaccagggaa ccgagcgcca 4681 cctcatgcac ctggaattgg acatctcgga ctccaaaatc aggtatgaat ctggggacca 4741 cgtggctgtg tacccagcca acgactctgc tctcgtcaac cagctgggca aaatcctggg 4801 tgccgacctg gacgtcgtca tgtccctgaa caacctggat gaggagtcca acaagaagca 4861 cccattcccg tgccctacgt cctaccgcac ggccctcacc tactacctgg acatcaccaa 4921 cccgccgcgt accaacgtgc tgtacgagct ggcgcagtac gcctcggagc cctcggagca 4981 ggagctgctg cgcaagatgg cctcctcctc cggcgagggc aaggagctgt acctgagctg 5041 ggtggtggag gcccggaggc acatcctggc catcctgcag gactgcccgt ccctgcggcc 5101 ccccatcgac cacctgtgtg agctgctgcc gcgcctgcag gcccgctact actccatcgc 5161 ctcatcctcc aaggtccacc ccaactctgt gcacatctgt gcggtggttg tggagtacga 5221 gaccaaggct ggccgcatca acaagggcgt ggccaccaac tggctgcggg ccaaggagcc 5281 tgccggggag aacggcggcc gtgcgctggt gcccatgttc gtgcgcaagt cccagttccg 5341 cctgcccttc aaggccacca cgcctgtcat catggtgggc cccggcaccg gggtggcacc 5401 cttcataggc ttcatccagg agcgggcctg gctgcgacag cagggcaagg aggtggggga 5461 gacgctgctg tactacggct gccgccgctc ggatgaggac tacctgtacc gggaggagct 5521 ggcgcagttc cacagggacg gtgcgctcac ccagctcaac gtggccttct cccgggagca 5581 gtcccacaag gtctacgtcc agcacctgct aaagcaagac cgagagcacc tgtggaagtt 5641 gatcgaaggc ggtgcccaca tctacgtctg tggggatgca cggaacatgg ccagggatgt 5701 gcagaacacc ttctacgaca tcgtggctga gctcggggcc atggagcacg cgcaggcggt 5761 ggactacatc aagaaactga tgaccaaggg ccgctactcc ctggacgtgt ggagcGTCGA 5821 CATGGAACAG AAGTTGATTT CCGAAGAAGA CCTCGAGTAA GCTTGGTACC GCGGCTAGCT 5881 AAGATCCGCT CTAACCGAAA AGGAAGGAGT TAGACAACCT GAAGTCTAGG TCCCTATTTA 5941 TTTTTTTATA GTTATGTTAG TATTAAGAAC GTTATTTATA TTTCAAATTT TTCTTTTTTT 6001 TCTGTACAGA CGCGTGTACG CATGTAACAT TATACTGAAA ACCTTGCTTG AGAAGGTTTT 6061 GGGACGCTCG AAGATCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT 6121 GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT 6181 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 6241 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 6301 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 6361 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 6421 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 6481 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 6541 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 6601 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 6661 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 6721 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT 6781 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 6841 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 6901 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 6961 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 7021 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA 7081 ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC 7141 CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC 7201 TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC 7261 AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT 7321 TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT 7381 TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC 7441 CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG 7501 CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT 7561 TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC 7621 TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG 7681 CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT 7741 TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC 7801 GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC 7861 TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA 7921 ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 7981 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 8041 CACATTTCCC CGAAAAGTGC CACCTGAACG AAGCATCTGT GCTTCATTTT GTAGAACAAA 8101 AATGCAACGC GAGAGCGCTA ATTTTTCAAA CAAAGAATCT GAGCTGCATT TTTACAGAAC 8161 AGAAATGCAA CGCGAAAGCG CTATTTTACC AACGAAGAAT CTGTGCTTCA TTTTTGTAAA 8221 ACAAAAATGC AACGCGAGAG CGCTAATTTT TCAAACAAAG AATCTGAGCT GCATTTTTAC 8281 AGAACAGAAA TGCAACGCGA GAGCGCTATT TTACCAACAA AGAATCTATA CTTCTTTTTT 8341 GTTCTACAAA AATGCATCCC GAGAGCGCTA TTTTTCTAAC AAAGCATCTT AGATTACTTT 8401 TTTTCTCCTT TGTGCGCTCT ATAATGCAGT CTCTTGATAA CTTTTTGCAC TGTAGGTCCG 8461 TTAAGGTTAG AAGAAGGCTA CTTTGGTGTC TATTTTCTCT TCCATAAAAA AAGCCTGACT 8521 CCACTTCCCG CGTTTACTGA TTACTAGCGA AGCTGCGGGT GCATTTTTTC AAGATAAAGG 8581 CATCCCCGAT TATATTCTAT ACCGATGTGG ATTGCGCATA CTTTGTGAAC AGAAAGTGAT 8641 AGCGTTGATG ATTCTTCATT GGTCAGAAAA TTATGAACGG TTTCTTCTAT TTTGTCTCTA 8701 TATACTACGT ATAGGAAATG TTTACATTTT CGTATTGTTT TCGATTCACT CTATGAATAG 8761 TTCTTACTAC AATTTTTTTG TCTAAAGAGT AATACTAGAG ATAAACATAA AAAATGTAGA 8821 GGTCGAGTTT AGATGCAAGT TCAAGGAGCG AAAGGTGGAT GGGTAGGTTA TATAGGGATA 8881 TAGCACAGAG ATATATAGCA AAGAGATACT TTTGAGCAAT GTTTGTGGAA GCGGTATTCG 8941 CAATATTTTA GTAGCTCGTT ACAGTCCGGT GCGTTTTTGG TTTTTTGAAA GTGCGTCTTC 9001 AGAGCGCTTT TGGTTTTCAA AAGCGCTCTG AAGTTCCTAT ACTTTCTAGA GAATAGGAAC 9061 TTCGGAATAG GAACTTCAAA GCGTTTCCGA AAACGAGCGC TTCCGAAAAT GCAACGCGAG 9121 CTGCGCACAT ACAGCTCACT GTTCACGTCG CACCTATATC TGCGTGTTGC CTGTATATAT 9181 ATATACATGA GAAGAACGGC ATAGTGCGTG TTTATGCTTA AATGCGTACT TATATGCGTC 9241 TATTTATGTA GGATGAAAGG TAGTCTAGTA CCTCCTGTGA TATTATCCCA TTCCATGCGG 9301 GGTATCGTAT GCTTCCTTCA GCACTACCCT TTAGCTGTTC TATATGCTGC CACTCCTCAA 9361 TTGGATTAGT CTCATCCTTC AATGCTATCA TTTCCTTTGA TATTGGATCA TACTAAGAAA 9421 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.