pLV3-CMV-EGFR-T790M-6×His-Puro

pLV3-CMV-EGFR-T790M-6×His-Puro plasmid

PVT109380

pLV3-CMV-EGFR-T790M-6×His-Puro plasmid Information

Plasmid name: pLV3-CMV-EGFR-T790M-6×His-Puro
Alias:
Gene lenght: 3657
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species:
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-EGFR-T790M-6×His-Puro plasmid Description

pLV3-CMV-EGFR-T790M-6×His-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-EGFR-T790M-6×His-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCgccac cATGCGACCC TCCGGGACGG CCGGGGCAGC GCTCCTGGCG 2341 CTGCTGGCTG CGCTCTGCCC GGCGAGTCGG GCTCTGGAGG AAAAGAAAGT TTGCCAAGGC 2401 ACGAGTAACA AGCTCACGCA GTTGGGCACT TTTGAAGATC ATTTTCTCAG CCTCCAGAGG 2461 ATGTTCAATA ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT GCAGAGGAAT 2521 TATGATCTTT CCTTCTTAAA GACCATCCAG GAGGTGGCTG GTTATGTCCT CATTGCCCTC 2581 AACACAGTGG AGCGAATTCC TTTGGAAAAC CTGCAGATCA TCAGAGGAAA TATGTACTAC 2641 GAAAATTCCT ATGCCTTAGC AGTCTTATCT AACTATGATG CAAATAAAAC CGGACTGAAG 2701 GAGCTGCCCA TGAGAAATTT ACAGGAAATC CTGCATGGCG CCGTGCGGTT CAGCAACAAC 2761 CCTGCCCTGT GCAACGTGGA GAGCATCCAG TGGCGGGACA TAGTCAGCAG TGACTTTCTC 2821 AGCAACATGT CGATGGACTT CCAGAACCAC CTGGGCAGCT GCCAAAAGTG TGATCCAAGC 2881 TGTCCCAATG GGAGCTGCTG GGGTGCAGGA GAGGAGAACT GCCAGAAACT GACCAAAATC 2941 ATCTGTGCCC AGCAGTGCTC CGGGCGCTGC CGTGGCAAGT CCCCCAGTGA CTGCTGCCAC 3001 AACCAGTGTG CTGCAGGCTG CACAGGCCCC CGGGAGAGCG ACTGCCTGGT CTGCCGCAAA 3061 TTCCGAGACG AAGCCACGTG CAAGGACACC TGCCCCCCAC TCATGCTCTA CAACCCCACC 3121 ACGTACCAGA TGGATGTGAA CCCCGAGGGC AAATACAGCT TTGGTGCCAC CTGCGTGAAG 3181 AAGTGTCCCC GTAATTATGT GGTGACAGAT CACGGCTCGT GCGTCCGAGC CTGTGGGGCC 3241 GACAGCTATG AGATGGAGGA AGACGGCGTC CGCAAGTGTA AGAAGTGCGA AGGGCCTTGC 3301 CGCAAAGTGT GTAACGGAAT AGGTATTGGT GAATTTAAAG ACTCACTCTC CATAAATGCT 3361 ACGAATATTA AACACTTCAA AAACTGCACC TCCATCAGTG GCGATCTCCA CATCCTGCCG 3421 GTGGCATTTA GGGGTGACTC CTTCACACAT ACTCCTCCTC TGGATCCACA GGAACTGGAT 3481 ATTCTGAAAA CCGTAAAGGA AATCACAGGG TTTTTGCTGA TTCAGGCTTG GCCTGAAAAC 3541 AGGACGGACC TCCATGCCTT TGAGAACCTA GAAATCATAC GCGGCAGGAC CAAGCAACAT 3601 GGTCAGTTTT CTCTTGCAGT CGTCAGCCTG AACATAACAT CCTTGGGATT ACGCTCCCTC 3661 AAGGAGATAA GTGATGGAGA TGTGATAATT TCAGGAAACA AAAATTTGTG CTATGCAAAT 3721 ACAATAAACT GGAAAAAACT GTTTGGGACC TCCGGTCAGA AAACCAAAAT TATAAGCAAC 3781 AGAGGTGAAA ACAGCTGCAA GGCCACAGGC CAGGTCTGCC ATGCCTTGTG CTCCCCCGAG 3841 GGCTGCTGGG GCCCGGAGCC CAGGGACTGC GTCTCTTGCC GGAATGTCAG CCGAGGCAGG 3901 GAATGCGTGG ACAAGTGCAA CCTTCTGGAG GGTGAGCCAA GGGAGTTTGT GGAGAACTCT 3961 GAGTGCATAC AGTGCCACCC AGAGTGCCTG CCTCAGGCCA TGAACATCAC CTGCACAGGA 4021 CGGGGACCAG ACAACTGTAT CCAGTGTGCC CACTACATTG ACGGCCCCCA CTGCGTCAAG 4081 ACCTGCCCGG CAGGAGTCAT GGGAGAAAAC AACACCCTGG TCTGGAAGTA CGCAGACGCC 4141 GGCCATGTGT GCCACCTGTG CCATCCAAAC TGCACCTACG GATGCACTGG GCCAGGTCTT 4201 GAAGGCTGTC CAACGAATGG GCCTAAGATC CCGTCCATCG CCACTGGGAT GGTGGGGGCC 4261 CTCCTCTTGC TGCTGGTGGT GGCCCTGGGG ATCGGCCTCT TCATGCGAAG GCGCCACATC 4321 GTTCGGAAGC GCACGCTGCG GAGGCTGCTG CAGGAGAGGG AGCTTGTGGA GCCTCTTACA 4381 CCCAGTGGAG AAGCTCCCAA CCAAGCTCTC TTGAGGATCT TGAAGGAAAC TGAATTCAAA 4441 AAGATCAAAG TGCTGGGCTC CGGTGCGTTC GGCACGGTGT ATAAGGGACT CTGGATCCCA 4501 GAAGGTGAGA AAGTTAAAAT TCCCGTCGCT ATCAAGGAAT TAAGAGAAGC AACATCTCCG 4561 AAAGCCAACA AGGAAATCCT CGATGAAGCC TACGTGATGG CCAGCGTGGA CAACCCCCAC 4621 GTGTGCCGCC TGCTGGGCAT CTGCCTCACC TCCACCGTGC AACTCATCAT GCAGCTCATG 4681 CCCTTCGGCT GCCTCCTGGA CTATGTCCGG GAACACAAAG ACAATATTGG CTCCCAGTAC 4741 CTGCTCAACT GGTGTGTGCA GATCGCAAAG GGCATGAACT ACTTGGAGGA CCGTCGCTTG 4801 GTGCACCGCG ACCTGGCAGC CAGGAACGTA CTGGTGAAAA CACCGCAGCA TGTCAAGATC 4861 ACAGATTTTG GGCTGGCCAA ACTGCTGGGT GCGGAAGAGA AAGAATACCA TGCAGAAGGA 4921 GGCAAAGTGC CTATCAAGTG GATGGCATTG GAATCAATTT TACACAGAAT CTATACCCAC 4981 CAGAGTGATG TCTGGAGCTA CGGGGTGACC GTTTGGGAGT TGATGACCTT TGGATCCAAG 5041 CCATATGACG GAATCCCTGC CAGCGAGATC TCCTCCATCC TGGAGAAAGG AGAACGCCTC 5101 CCTCAGCCAC CCATATGTAC CATCGATGTC TACATGATCA TGGTCAAGTG CTGGATGATA 5161 GACGCAGATA GTCGCCCAAA GTTCCGTGAG TTGATCATCG AATTCTCCAA AATGGCCCGA 5221 GACCCCCAGC GCTACCTTGT CATTCAGGGG GATGAAAGAA TGCATTTGCC AAGTCCTACA 5281 GACTCCAACT TCTACCGTGC CCTGATGGAT GAAGAAGACA TGGACGACGT GGTGGATGCC 5341 GACGAGTACC TCATCCCACA GCAGGGCTTC TTCAGCAGCC CCTCCACGTC ACGGACTCCC 5401 CTCCTGAGCT CTCTGAGTGC AACCAGCAAC AATTCCACCG TGGCTTGCAT TGATAGAAAT 5461 GGGCTGCAAA GCTGTCCCAT CAAGGAAGAC AGCTTCTTGC AGCGATACAG CTCAGACCCC 5521 ACAGGCGCCT TGACTGAGGA CAGCATAGAC GACACCTTCC TCCCAGTGCC TGAATACATA 5581 AACCAGTCCG TTCCCAAAAG GCCCGCTGGC TCTGTGCAGA ATCCTGTCTA TCACAATCAG 5641 CCTCTGAACC CCGCGCCCAG CAGAGACCCA CACTACCAGG ACCCCCACAG CACTGCAGTG 5701 GGCAACCCCG AGTATCTCAA CACTGTCCAG CCCACCTGTG TCAACAGCAC ATTCGACAGC 5761 CCTGCCCACT GGGCCCAGAA AGGCAGCCAC CAAATTAGCC TGGACAACCC TGACTACCAG 5821 CAGGACTTCT TTCCCAAGGA AGCCAAGCCA AATGGCATCT TTAAGGGCTC CACAGCTGAA 5881 AATGCAGAAT ACCTAAGGGT CGCGCCACAA AGCAGTGAAT TTATTGGAGC AGGATCCCAT 5941 CACCATCACC ATCACTAGCG GCCGCGAAGG ATCTGCGATC GCTCCGGTGC CCGTCAGTGG 6001 GCAGAGCGCA CATCGCCCAC AGTCCCCGAG AAGTTGGGGG GAGGGGTCGG CAATTGAACG 6061 GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC 6121 CTTTTTCCCG AGGGTGGGGG AGAACCGTAT ATAAGTGCAG TAGTCGCCGT GAACGTTCTT 6181 TTTCGCAACG GGTTTGCCGC CAGAACACAG CTGAAGCTTC GAGGGGCTCG CATCTCTCCT 6241 TCACGCGCCC GCCGCCCTAC CTGAGGCCGC CATCCACGCC GGTTGAGTCG CGTTCTGCCG 6301 CCTCCCGCCT GTGGTGCCTC CTGAACTGCG TCCGCCGTCT AGGTAAGTTT AAAGCTCAGG 6361 TCGAGACCGG GCCTTTGTCC GGCGCTCCCT TGGAGCCTAC CTAGACTCAG CCGGCTCTCC 6421 ACGCTTTGCC TGACCCTGCT TGCTCAACTC TACGTCTTTG TTTCGTTTTC TGTTCTGCGC 6481 CGTTACAGAT CCAAGCTGTG ACCGGCGCCT ACGCTAGATG ACCGAGTACA AGCCCACGGT 6541 GCGCCTCGCC ACCCGCGACG ACGTCCCCCG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC 6601 CGACTACCCC GCCACGCGCC ACACCGTCGA CCCGGACCGC CACATCGAGC GGGTCACCGA 6661 GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA 6721 CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT 6781 CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA 6841 GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT 6901 CGGCGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT 6961 GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT 7021 CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC 7081 GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGAGTC GACAATCAAC CTCTGGATTA 7141 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 7201 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 7261 CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG TTGTGGCCCG TTGTCAGGCA 7321 ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC ACTGGTTGGG GCATTGCCAC 7381 CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC CCTATTGCCA CGGCGGAACT 7441 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC 7501 CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG CTCGCCTGTG TTGCCACCTG 7561 GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC CTCAATCCAG CGGACCTTCC 7621 TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC 7681 GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCCTGGTACC TTTAAGACCA ATGACTTACA 7741 AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC 7801 ACTCCCAACG AAAATAAGAT CTGCTTTTTG CTTGTACTGG GTCTCTCTGG TTAGACCAGA 7861 TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT 7921 TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT 7981 CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG TAGTAGTTCA TGTCATCTTA 8041 TTATTCAGTA TTTATAACTT GCAAAGAAAT GAATATCAGA GAGTGAGAGG AACTTGTTTA 8101 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 8161 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 8221 GGCTCTAGCT ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG 8281 ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG GCCTCTGAGC TATTCCAGAA 8341 GTAGTGAGGA GGCTTTTTTG GAGGCCTAGA CTTTTGCAGA GACGGCCCAA ATTCGTAATC 8401 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG 8461 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT 8521 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG 8581 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT 8641 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 8701 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 8761 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 8821 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 8881 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 8941 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 9001 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 9061 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 9121 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 9181 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 9241 TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 9301 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 9361 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 9421 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 9481 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 9541 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC 9601 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT 9661 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC 9721 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC 9781 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG 9841 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG 9901 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG 9961 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG 10021 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT 10081 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA 10141 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC 10201 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC 10261 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC 10321 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC 10381 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA 10441 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT 10501 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT 10561 CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT 10621 TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC 10681 GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC 10741 GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG 10801 AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG 10861 GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC 10921 GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC 10981 AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GCCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.