pLV2-ARHGAP22-3×FLAG-IRES-Puro

pLV2-ARHGAP22-3×FLAG-IRES-Puro plasmid

PVT106682

pLV2-ARHGAP22-3×FLAG-IRES-Puro plasmid Information

Plasmid name: pLV2-ARHGAP22(human)-3×FLAG-IRES-Puro
Alias: NM_001256024.2;RhoGAP2; RhoGap22
Gene lenght: 2142
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-ARHGAP22-3×FLAG-IRES-Puro plasmid Description

pLV2-ARHGAP22-3×FLAG-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and is also applicable for lentivirus packaging.

pLV2-ARHGAP22-3×FLAG-IRES-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatgctg 2821 agcccaaaga tcaggcaggc caggagggcc cgctccaaaa gcctagtgat gggggagcag 2881 agccggagcc ctgggcggat gccgtgccct cacaggctgg gccccgtgct gaaggcgggc 2941 tggctgaaga agcagaggag catcatgaag aactggcagc agcgctggtt tgtgctgcgt 3001 ggggatcagc ttttctacta caaggacaaa gatgagatca agccccaggg atttatttct 3061 ctacaaggga cacaggtgac tgaacttcct cctggccccg aggacccagg gaagcacctc 3121 tttgagatca gcccaggtgg tgccggggag cgggagaagg tgccggccaa ccccgaggcg 3181 ctcctgctca tggccagctc ccagcgtgac atggaggact gggtgcaggc catccgccga 3241 gtcatctggg ccccgctggg cggaggtact gcccgtagat cgcatgccca ccctctagaa 3301 cccttgcctc cagggatctt tgggcagcgc ctagaggaaa cagtccacca cgagcggaag 3361 tatggccccc gcctggcgcc cctgctggtg gagcagtgtg tggacttcat ccgggagcgc 3421 gggctcactg aggaggggct gttccgcatg ccaggccagg ccaacctggt gagggacctg 3481 caggattcct tcgactgtgg ggagaagcca ctgtttgaca gcacaacaga cgtgcacacg 3541 gtggcctccc tgctgaagct gtacctgcgg gagctccccg agcccgtggt ccccttcgcc 3601 aggtacgagg acttcctcag ctgcgcccag ctgctcacca aggacgaggg ggagggcact 3661 ctggagttgg ctaaacaagt gagcaacctt cctcaggcaa attacaacct gctcagatac 3721 atctgcaagt ttctggatga agttcaggca tactcaaatg tcaacaagat gagtgtccag 3781 aatctggcaa ccgtttttgg acctaacatt ctgcggccac aggtagagga cccagtaacc 3841 atcatggaag gcacttccct cgtccagcac ctgatgaccg tcctcatccg caaacacagc 3901 cagctcttca cggcaccggt cccggaaggg cccacctccc cgcgcggggg cctgcaatgc 3961 gcagtggggt ggggctccga ggaggtcacc agggacagcc aaggagagcc cggcggcccc 4021 ggcctgcccg cgcacaggac ctcttccctg gacggggcgg ccgtggcggt gctctccaga 4081 acagccccca cggggccggg gagccggtgc agccctggga agaaggtgca gaccctgccc 4141 agttggaagt cctccttccg gcagccgagg tccctatcgg gaagcccgaa ggggggcggc 4201 tcatccctgg aggtgcccat catctcctcc ggcgggaact ggcttatgaa cgggctgtcc 4261 tccctgcgcg gacaccgccg ggcctcgtcg ggagaccggc tcaaggactc gggctccgtg 4321 cagagactct ccacctacga caatgtgccc gcgccgggcc tggtccccgg catacccagc 4381 gtggccagta tggcgtggtc cggggcctcg tccagcgagt cgtcggtggg gggctcactc 4441 agcagctgca cggcctgccg cgccagcgac tcgtctgccc gcagttccct gcacaccgac 4501 tgggccctgg agccctcccc gctccccagc agcagcgagg accccaagtc cctggacctg 4561 gaccacagca tggacgaggc gggcgcgggt gccagcaaca gcgagcccag cgagccggac 4621 agccccaccc gggaacacgc gcgccgctcc gaggccttac aggggctggt cactgagctc 4681 agggccgagc tgtgccgcca gcggactgag tacgagagga gtgtgaaaag aatcgaagaa 4741 gggagtgctg acctgagaaa acgaatgtcc cggttagaag aagaactgga ccaggaaaag 4801 aaaaaataca tcatgctgga aataaagctg cggaactctg aacgggcgcg ggaggatgcg 4861 gagaggagga accagctgtt gcagagggaa atggaggagt ttttttcgac cctaggaagc 4921 ttgactgttg gggcaaaagg tgccagggcc ccaaagctcg agGACTACAA AGACCATGAC 4981 GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA Ggatcccgcc 5041 cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg 5101 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga 5161 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa 5221 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa 5281 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct 5341 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg 5401 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg 5461 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca 5521 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg 5581 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccacaa ggagacgacc 5641 ttccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc 5701 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc 5761 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct 5821 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc 5881 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag 5941 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa 6001 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct 6061 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt 6121 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac 6181 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc 6241 ctagacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 6301 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 6361 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 6421 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 6481 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 6541 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 6601 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 6661 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 6721 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 6781 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 6841 ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa tcacaagtag 6901 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt 6961 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga 7021 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag 7081 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa 7141 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt 7201 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca 7261 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga 7321 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg 7381 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct 7441 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct 7501 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 7561 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 7621 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 7681 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa 7741 tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct agctagagct 7801 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 7861 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 7921 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 7981 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 8041 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 8101 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 8161 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 8221 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 8281 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 8341 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 8401 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 8461 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 8521 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 8581 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 8641 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 8701 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 8761 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 8821 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 8881 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 8941 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 9001 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 9061 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 9121 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 9181 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 9241 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 9301 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 9361 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 9421 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 9481 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 9541 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 9601 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 9661 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 9721 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 9781 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 9841 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 9901 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 9961 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa 10021 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 10081 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct 10141 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg 10201 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt 10261 tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta 10321 ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.