pPIC9K-α-factor-HMOX1 plasmid

pPIC9K-α-factor-HMOX1 plasmid

PVT111513

pPIC9K-α-factor-HMOX1 plasmid Information

Plasmid name: pPIC9K-α-factor-HMOX1(human)
Alias: NM_002133.3;bK286B10; HMOX1D; HO-1; HSP32
Gene lenght: 867
Host: Yeast
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: HIS4
Fluorescent labeling:
Promoter: AOX1
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: α-factor-F1（HighGC）
Reverse primer:
Copy number: High
Induction:

pPIC9K-α-factor-HMOX1 plasmid Description

pPIC9K-α-factor-HMOX1 is a plasmid suitable for gene expression in yeast.

pPIC9K-α-factor-HMOX1 plasmid Sequence

1 agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 61 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 121 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 181 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 241 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 301 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 361 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 421 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 481 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 541 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 601 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 661 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 721 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 781 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 841 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 901 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct 961 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact 1021 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta 1081 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg 1141 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa 1201 agagaggctg aagcttacgt agaattcatg gagcgtccgc aacccgacag catgccccag 1261 gatttgtcag aggccctgaa ggaggccacc aaggaggtgc acacccaggc agagaatgct 1321 gagttcatga ggaactttca gaagggccag gtgacccgag acggcttcaa gctggtgatg 1381 gcctccctgt accacatcta tgtggccctg gaggaggaga ttgagcgcaa caaggagagc 1441 ccagtcttcg cccctgtcta cttcccagaa gagctgcacc gcaaggctgc cctggagcag 1501 gacctggcct tctggtacgg gccccgctgg caggaggtca tcccctacac accagccatg 1561 cagcgctatg tgaagcggct ccacgaggtg gggcgcacag agcccgagct gctggtggcc 1621 cacgcctaca cccgctacct gggtgacctg tctgggggcc aggtgctcaa aaagattgcc 1681 cagaaagccc tggacctgcc cagctctggc gagggcctgg ccttcttcac cttccccaac 1741 attgccagtg ccaccaagtt caagcagctc taccgctccc gcatgaactc cctggagatg 1801 actcccgcag tcaggcagag ggtgatagaa gaggccaaga ctgcgttcct gctcaacatc 1861 cagctctttg aggagttgca ggagctgctg acccatgaca ccaaggacca gagcccctca 1921 cgggcaccag ggcttcgcca gcgggccagc aacaaagtgc aagattctgc ccccgtggag 1981 actcccagag ggaagccccc actcaacacc cgctcccagg ctccgcttct ccgatgggtc 2041 cttacactca gctttctggt ggcgacagtt gctgtagggc tttatgccat gtgagcggcc 2101 gcgaattaat tcgccttaga catgactgtt cctcagttca agttgggcac ttacgagaag 2161 accggtcttg ctagattcta atcaagagga tgtcagaatg ccatttgcct gagagatgca 2221 ggcttcattt ttgatacttt tttatttgta acctatatag tataggattt tttttgtcat 2281 tttgtttctt ctcgtacgag cttgctcctg atcagcctat ctcgcagctg atgaatatct 2341 tgtggtaggg gtttgggaaa atcattcgag tttgatgttt ttcttggtat ttcccactcc 2401 tcttcagagt acagaagatt aagtgagaag ttcgtttgtg caagcttatc gataagcttt 2461 aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta 2521 acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg 2581 ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc 2641 actatggcgt gctgctagcg ctatatgcgt tgatgcaatt tctatgcgca cccgttctcg 2701 gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct cgcttcgcta cttggagcca 2761 ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg gatctatcga atctaaatgt 2821 aagttaaaat ctctaaataa ttaaataagt cccagtttct ccatacgaac cttaacagca 2881 ttgcggtgag catctagacc ttcaacagca gccagatcca tcactgcttg gccaatatgt 2941 ttcagtccct caggagttac gtcttgtgaa gtgatgaact tctggaaggt tgcagtgtta 3001 actccgctgt attgacgggc atatccgtac gttggcaaag tgtggttggt accggaggag 3061 taatctccac aactctctgg agagtaggca ccaacaaaca cagatccagc gtgttgtact 3121 tgatcaacat aagaagaagc attctcgatt tgcaggatca agtgttcagg agcgtactga 3181 ttggacattt ccaaagcctg ctcgtaggtt gcaaccgata gggttgtaga gtgtgcaata 3241 cacttgcgta caatttcaac ccttggcaac tgcacagctt ggttgtgaac agcatcttca 3301 attctggcaa gctccttgtc tgtcatatcg acagccaaca gaatcacctg ggaatcaata 3361 ccatgttcag cttgagacag aaggtctgag gcaacgaaat ctggatcagc gtatttatca 3421 gcaataacta gaacttcaga aggcccagca ggcatgtcaa tactacacag ggctgatgtg 3481 tcattttgaa ccatcatctt ggcagcagta acgaactggt ttcctggacc aaatattttg 3541 tcacacttag gaacagtttc tgttccgtaa gccatagcag ctactgcctg ggcgcctcct 3601 gctagcacga tacacttagc accaaccttg tgggcaacgt agatgacttc tggggtaagg 3661 gtaccatcct tcttaggtgg agatgcaaaa acaatttctt tgcaaccagc aactttggca 3721 ggaacaccca gcatcaggga agtggaaggc agaattgcgg ttccaccagg aatatagagg 3781 ccaactttct caataggtct tgcaaaacga gagcagacta caccagggca agtctcaact 3841 tgcaacgtct ccgttagttg agcttcatgg aatttcctga cgttatctat agagagatca 3901 atggctctct taacgttatc tggcaattgc ataagttcct ctgggaaagg agcttctaac 3961 acaggtgtct tcaaagcgac tccatcaaac ttggcagtta gttctaaaag ggctttgtca 4021 ccattttgac gaacattgtc gacaattggt ttgactaatt ccataatctg ttccgttttc 4081 tggataggac gacgaagggc atcttcaatt tcttgtgagg aggccttaga aacgtcaatt 4141 ttgcacaatt caatacgacc ttcagaaggg acttctttag gtttggattc ttctttaggt 4201 tgttccttgg tgtatcctgg cttggcatct cctttccttc tagtgacctt tagggacttc 4261 atatccaggt ttctctccac ctcgtccaac gtcacaccgt acttggcaca tctaactaat 4321 gcaaaataaa ataagtcagc acattcccag gctatatctt ccttggattt agcttctgca 4381 agttcatcag cttcctccct aattttagcg ttcaacaaaa cttcgtcgtc aaataaccgt 4441 ttggtataag aaccttctgg agcattgctc ttacgatccc acaaggtggc ttccatggct 4501 ctaagaccct ttgattggcc aaaacaggaa gtgcgttcca agtgacagaa accaacacct 4561 gtttgttcaa ccacaaattt caagcagtct ccatcacaat ccaattcgat acccagcaac 4621 ttttgagttg ctccagatgt agcaccttta taccacaaac cgtgacgacg agattggtag 4681 actccagttt gtgtccttat agcctccgga atagactttt tggacgagta caccaggccc 4741 aacgagtaat tagaagagtc agccaccaaa gtagtgaata gaccatcggg gcggtcagta 4801 gtcaaagacg ccaacaaaat ttcactgaca gggaactttt tgacatcttc agaaagttcg 4861 tattcagtag tcaattgccg agcatcaata atggggatta taccagaagc aacagtggaa 4921 gtcacatcta ccaactttgc ggtctcagaa aaagcataaa cagttctact accgccatta 4981 gtgaaacttt tcaaatcgcc cagtggagaa gaaaaaggca cagcgatact agcattagcg 5041 ggcaaggatg caactttatc aaccagggtc ctatagataa ccctagcgcc tgggatcatc 5101 ctttggacaa ctctttctgc caaatctagg tccaaaatca cttcattgat accattattg 5161 tacaacttga gcaagttgtc gatcagctcc tcaaattggt cctctgtaac ggatgactca 5221 acttgcacat taacttgaag ctcagtcgat tgagtgaact tgatcaggtt gtgcagctgg 5281 tcagcagcat agggaaacac ggcttttcct accaaactca aggaattatc aaactctgca 5341 acacttgcgt atgcaggtag caagggaaat gtcatacttg aagtcggaca gtgagtgtag 5401 tcttgagaaa ttctgaagcc gtatttttat tatcagtgag tcagtcatca ggagatcctc 5461 tacgccggac gcatcgtggc cgacctgcag gggggggggg ggcgctgagg tctgcctcgt 5521 gaagaaggtg ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg 5581 agggagccac ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt 5641 tgctttgcca cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca 5701 gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta atgctctgcc 5761 agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact 5821 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg 5881 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga 5941 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat 6001 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca 6061 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat 6121 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt 6181 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat 6241 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg 6301 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg 6361 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg 6421 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc 6481 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat 6541 cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc 6601 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata 6661 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg ctttcccccc 6721 cccccctgca ggtcggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg 6781 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg 6841 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac 6901 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc 6961 aggagtcgca taagggagag cgtcgagtat ctatgattgg aagtatggga atggtgatac 7021 ccgcattctt cagtgtcttg aggtctccta tcagattatg cccaactaaa gcaaccggag 7081 gaggagattt catggtaaat ttctctgact tttggtcatc agtagactcg aactgtgaga 7141 ctatctcggt tatgacagca gaaatgtcct tcttggagac agtaaatgaa gtcccaccaa 7201 taaagaaatc cttgttatca ggaacaaact tcttgtttcg aactttttcg gtgccttgaa 7261 ctataaaatg tagagtggat atgtcgggta ggaatggagc gggcaaatgc ttaccttctg 7321 gaccttcaag aggtatgtag ggtttgtaga tactgatgcc aacttcagtg acaacgttgc 7381 tatttcgttc aaaccattcc gaatccagag aaatcaaagt tgtttgtcta ctattgatcc 7441 aagccagtgc ggtcttgaaa ctgacaatag tgtgctcgtg ttttgaggtc atctttgtat 7501 gaataaatct agtctttgat ctaaataatc ttgacgagcc aaggcgataa atacccaaat 7561 ctaaaactct tttaaaacgt taaaaggaca agtatgtctg cctgtattaa accccaaatc 7621 agctcgtagt ctgatcctca tcaacttgag gggcactatc ttgttttaga gaaatttgcg 7681 gagatgcgat atcgagaaaa aggtacgctg attttaaacg tgaaatttat ctcaagatct 7741 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 7801 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 7861 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 7921 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt 7981 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg 8041 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 8101 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 8161 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 8221 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 8281 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 8341 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 8401 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 8461 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 8521 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 8581 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 8641 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 8701 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 8761 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 8821 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 8881 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 8941 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 9001 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 9061 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 9121 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 9181 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 9241 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca 9301 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 9361 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 9421 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 9481 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 9541 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg 9601 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 9661 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 9721 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 9781 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 9841 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 9901 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 9961 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 10021 tttcgtcttc aagaattaat tctcatgttt gacagcttat catcgataag ctgactcatg 10081 ttggtattgt gaaatagacg cagatcggga acactgaaaa ataacagtta ttattcg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.