pLV3-EF1a-NLS-MCS-3×FLAG-PGK-P

pLV3-EF1a-NLS-MCS-3×FLAG-PGK-Puro plasmid

PVT99861

pLV3-EF1a-NLS-MCS-3×FLAG-PGK-Puro plasmid Information

Plasmid name: pLV3-EF1a-NLS-MCS-3×FLAG-PGK-Puro
Alias: -
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression,subcellular localization
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-NLS-MCS-3×FLAG-PGK-Puro plasmid Description

pLV3-EF1a-NLS-MCS-3×FLAG-PGK-Puro is a plasmid suitable for protein expression and subcellular localization in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-NLS-MCS-3×FLAG-PGK-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cgccaccatg CCAAAAAAGA AGAGAAAGGT 2521 ACTCGAGgga tccGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA 2581 CAAGGATGAC GATGACAAGt agcggccgct gggtagggga ggcgcttttc ccaaggcagt 2641 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc 2701 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct 2761 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt 2821 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca 2881 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct 2941 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc 3001 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa 3061 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc 3121 caagcttacc atggagagcg acgagagcgg cctgatgacc gagtacaagc ccacggtgcg 3181 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 3241 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 3301 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 3361 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 3421 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 3481 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 3541 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 3601 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 3661 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 3721 cacctggtgc atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa 3781 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata 3841 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc 3901 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg 3961 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac 4021 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat 4081 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt 4141 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat 4201 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc 4261 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag 4321 tcggatctcc ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg 4381 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact 4441 cccaacgaaa ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct 4501 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc 4561 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc 4621 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta 4681 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg 4741 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 4801 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc 4861 tctagctatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 4921 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 4981 gtgaggaggc ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg 5041 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 5101 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 5161 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 5221 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 5281 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 5341 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 5401 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 5461 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 5521 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 5581 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 5641 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 5701 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 5761 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 5821 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 5881 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 5941 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 6001 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 6061 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 6121 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 6181 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 6241 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 6301 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 6361 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 6421 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 6481 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 6541 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 6601 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 6661 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 6721 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 6781 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 6841 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 6901 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 6961 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 7021 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 7081 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 7141 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 7201 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 7261 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt 7321 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg 7381 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt 7441 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 7501 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 7561 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 7621 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.