pAOX1-α-factor-FCY1-EGFP-Myc-6

pAOX1-α-factor-FCY1-EGFP-Myc-6×His-Zeo plasmid

PVT111261

pAOX1-α-factor-FCY1-EGFP-Myc-6×His-Zeo plasmid Information

Plasmid name: pAOX1-α-factor-FCY1(yeast)-EGFP-Myc-6×His-Zeo
Alias:
Gene lenght: 474
Host: Yeast
Use(s): Gene expression
Fragment type: ORF
Species: Yeast
Prokaryotic resistance: Zeo
Screening marker: Zeo
Fluorescent labeling: Green
Promoter: AOX1
Replicon: pUC
Growth Strain(s): DH10B
Growth Temperature: 37°C
Vector backbone:
Forward primer: α-factor-F3
Reverse primer:
Copy number: Low
Induction:

pAOX1-α-factor-FCY1-EGFP-Myc-6×His-Zeo plasmid Description

pAOX1-α-factor-FCY1-EGFP-Myc-6×His-Zeo is a plasmid suitable for gene expression in yeast.

pAOX1-α-factor-FCY1-EGFP-Myc-6×His-Zeo plasmid Sequence

1 AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG 61 GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT 121 TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC 181 AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA 241 ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA 301 TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG 361 AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT 421 GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG 481 TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGGCA TACCGTTTGT 541 CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT 601 CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT 661 TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT 721 GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT 781 ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT 841 ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA 901 CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAACG ATGAGATTTC CTTCAATTTT 961 TACTGCTGTT TTATTCGCAG CATCCTCCGC ATTAGCTGCT CCAGTCAACA CTACAACAGA 1021 AGATGAAACG GCACAAATTC CGGCTGAAGC TGTCATCGGT TACTCAGATT TAGAAGGGGA 1081 TTTCGATGTT GCTGTTTTGC CATTTTCCAA CAGCACAAAT AACGGGTTAT TGTTTATAAA 1141 TACTACTATT GCCAGCATTG CTGCTAAAGA AGAAGGGGTA TCTCTCGAGA AAAGAGAGGC 1201 TGAAGCTGAA TTCatggtga cagggggaat ggcaagcaag tgggatcaga agggtatgga 1261 cattgcctat gaggaggcgg ccttaggtta caaagagggt ggtgttccta ttggcggatg 1321 tcttatcaat aacaaagacg gaagtgttct cggtcgtggt cacaacatga gatttcaaaa 1381 gggatccgcc acactacatg gtgagatctc cactttggaa aactgtggga gattagaggg 1441 caaagtgtac aaagatacca ctttgtatac gacgctgtct ccatgcgaca tgtgtacagg 1501 tgccatcatc atgtatggta ttccacgctg tgttgtcggt gagaacgtta atttcaaaag 1561 taagggcgag aaatatttac aaactagagg tcacgaggtt gttgttgttg acgatgagag 1621 gtgtaaaaag atcatgaaac aatttatcga tgaaagacct caggattggt ttgaagatat 1681 tggtgaggga tccGGCGGTG GAGGAAGCgg cggaggtgga tctatggtga gcaagggcga 1741 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 1801 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa 1861 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 1921 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 1981 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa 2041 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 2101 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta 2161 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt 2221 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa 2281 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc 2341 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac 2401 cgccgccggg atcactctcg gcatggacga gctgtacaag TCTCTAGAAC AAAAACTCAT 2461 CTCAGAAGAG GATCTGAATA GCGCCGTCGA CCATCATCAT CATCATCATT GAGTTTGTAG 2521 CCTTAGACAT GACTGTTCCT CAGTTCAAGT TGGGCACTTA CGAGAAGACC GGTCTTGCTA 2581 GATTCTAATC AAGAGGATGT CAGAATGCCA TTTGCCTGAG AGATGCAGGC TTCATTTTTG 2641 ATACTTTTTT ATTTGTAACC TATATAGTAT AGGATTTTTT TTGTCATTTT GTTTCTTCTC 2701 GTACGAGCTT GCTCCTGATC AGCCTATCTC GCAGCTGATG AATATCTTGT GGTAGGGGTT 2761 TGGGAAAATC ATTCGAGTTT GATGTTTTTC TTGGTATTTC CCACTCCTCT TCAGAGTACA 2821 GAAGATTAAG TGAGACCTTC GTTTGTGCGG ATCCCCCACA CACCATAGCT TCAAAATGTT 2881 TCTACTCCTT TTTTACTCTT CCAGATTTTC TCGGACTCCG CGCATCGCCG TACCACTTCA 2941 AAACACCCAA GCACAGCATA CTAAATTTCC CCTCTTTCTT CCTCTAGGGT GTCGTTAATT 3001 ACCCGTACTA AAGGTTTGGA AAAGAAAAAA GAGACCGCCT CGTTTCTTTT TCTTCGTCGA 3061 AAAAGGCAAT AAAAATTTTT ATCACGTTTC TTTTTCTTGA AATTTTTTTT TTTTGATTTT 3121 TTTCTCTTTC GATGACCTCC CATTGATATT TAAGTTAATA AACGGTCTTC AATTTCTCAA 3181 GTTTCAGTTT CATTTTTCTT GTTCTATTAC AACTTTTTTT ACTTCTTGTT CATTAGAAAG 3241 AAAGCATAGC AATCTAATCT AAGGGGCGGT GTTGACAATT AATCATCGGC ATAGTATATC 3301 GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT 3361 CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG 3421 TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG 3481 TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG 3541 CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC 3601 GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG 3661 CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTCCGA 3721 CGGCGGCCCA CGGGTCCCAG GCCTCGGAGA TCCGTCCCCC TTTTCCTTTG TCGATATCAT 3781 GTAATTAGTT ATGTCACGCT TACATTCACG CCCTCCCCCC ACATCCGCTC TAACCGAAAA 3841 GGAAGGAGTT AGACAACCTG AAGTCTAGGT CCCTATTTAT TTTTTTATAG TTATGTTAGT 3901 ATTAAGAACG TTATTTATAT TTCAAATTTT TCTTTTTTTT CTGTACAGAC GCGTGTACGC 3961 ATGTAACATT ATACTGAAAA CCTTGCTTGA GAAGGTTTTG GGACGCTCGA AGGCTTTAAT 4021 TTGCAAGCTG GAGACCAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 4081 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 4141 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 4201 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 4261 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC 4321 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 4381 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 4441 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 4501 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC 4561 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 4621 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 4681 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 4741 ACGTTAAGGG ATTTTGGTCA TGAGATC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.