pLV3-CMV-SMAD6-3×FLAG-CopGFP-P

pLV3-CMV-SMAD6-3×FLAG-CopGFP-Puro plasmid

PVT111278

pLV3-CMV-SMAD6-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SMAD6(human)-3×FLAG-CopGFP-Puro
Alias: NM_005585.5;AOVD2; HsT17432; MADH6; MADH7
Gene lenght: 1488
Host: Mammalian cells,lentivirus
Use(s): Gene expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SMAD6-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-SMAD6-3×FLAG-CopGFP-Puro It is a plasmid suitable for gene expression in mammalian cells, and is also suitable for lentivirus packaging.

pLV3-CMV-SMAD6-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgt tcaggtccaa acgctcgggg 2341 ctggtgcggc gactttggcg aagtcgtgtg gtccccgacc gggaggaagg cggcagcggc 2401 ggcggcggtg gcggcgacga ggatgggagc ttgggcagcc gagctgagcc ggccccgcgg 2461 gcaagagagg gcggaggctg cggccgctcc gaagtccgcc cggtagcccc gcggcggccc 2521 cgggacgcag tgggacagcg aggcgcccag ggcgcgggga ggcgccggcg cgcagggggc 2581 cccccgaggc ccatgtcgga gccaggggcc ggcgctggga gctccctgct ggacgtggcg 2641 gagccgggag gcccgggctg gctgcccgag agtgactgcg agacggtgac ctgctgtctc 2701 ttttcggagc gggacgccgc cggcgcgccc cgggacgcca gcgaccccct ggccggggcg 2761 gccctggagc cggcgggcgg cgggcggagt cgcgaagcgc gctcgcggct gctgctgctg 2821 gagcaggaac tcaaaaccgt cacgtactcg ctgctgaagc ggctcaagga gcgctcgctg 2881 gacacgctgc tggaggcggt ggagtcccgc ggcggcgtgc cgggcggctg cgtgctggtg 2941 ccgcgcgccg acctccgcct gggcggccag cccgcgccgc cgcagctgct gctcggccgc 3001 ctctttcgct ggcccgacct gcagcacgcc gtggagctga agcccctgtg cggctgccac 3061 agcttcgccg ccgccgccga cggccctacc gtgtgctgca acccctacca cttcagccgg 3121 ctctgcgggc ccgaatctcc gccacctccc tactctcggc tgtctcctcg cgacgagtac 3181 aagccactgg atctgtccga ttccacattg tcttacactg aaacggaggc taccaactcc 3241 ctcatcactg ctccgggtga attctcagac gccagcatgt ctccggacgc caccaagccg 3301 agccactggt gcagcgtggc gtactgggag caccggacgc gcgtgggccg cctctatgcg 3361 gtgtacgacc aggccgtcag catcttctac gacctacctc agggcagcgg cttctgcctg 3421 ggccagctca acctggagca gcgcagcgag tcggtgcggc gaacgcgcag caagatcggc 3481 ttcggcatcc tgctcagcaa ggagcccgac ggcgtgtggg cctacaaccg cggcgagcac 3541 cccatcttcg tcaactcccc gacgctggac gcgcccggcg gccgcgccct ggtcgtgcgc 3601 aaggtgcccc ccggctactc catcaaggtg ttcgacttcg agcgctcggg cctgcagcac 3661 gcgcccgagc ccgacgccgc cgacggcccc tacgacccca acagcgtccg catcagcttc 3721 gccaagggct gggggccctg ctactcccgg cagttcatca cctcctgccc ctgctggctg 3781 gagatcctcc tcaacaaccc cagaggatcc GACTACAAAG ACCATGACGG TGATTATAAA 3841 GATCATGACA TCGACTACAA GGATGACGAT GACAAGtagc ggccgcGaag gatctgcgat 3901 cgctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 3961 ggaggggtcg gcaattgaac gggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 4021 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 4081 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca gctgaagctt 4141 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 4201 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 4261 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 4321 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 4381 gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt gaccggcgcc tacgctagac 4441 gccaccatgg agagcgacga gagcggcctg cccgccatgg agatcgagtg ccgcatcacc 4501 ggcaccctga acggcgtgga gttcgagctg gtgggcggcg gagagggcac ccccaagcag 4561 ggccgcatga ccaacaagat gaagagcacc aaaggcgccc tgaccttcag cccctacctg 4621 ctgagccacg tgatgggcta cggcttctac cacttcggca cctaccccag cggctacgag 4681 aaccccttcc tgcacgccat caacaacggc ggctacacca acacccgcat cgagaagtac 4741 gaggacggcg gcgtgctgca cgtgagcttc agctaccgct acgaggccgg ccgcgtgatc 4801 ggcgacttca aggtggtggg caccggcttc cccgaggaca gcgtgatctt caccgacaag 4861 atcatccgca gcaacgccac cgtggagcac ctgcacccca tgggcgataa cgtgctggtg 4921 ggcagcttcg cccgcacctt cagcctgcgc gacggcggct actacagctt cgtggtggac 4981 agccacatgc acttcaagag cgccatccac cccagcatcc tgcagaacgg gggccccatg 5041 ttcgccttcc gccgcgtgga ggagctgcac agcaacaccg agctgggcat cgtggagtac 5101 cagcacgcct tcaagacccc catcgccttc gccagatccc gcgctcagtc gtccaattct 5161 gccgtggacg gcaccgccgg acccggctcc accggatctc gcgagggcag aggaagtctt 5221 ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa gcccacggtg 5281 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc 5341 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag 5401 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 5461 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 5521 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 5581 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 5641 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 5701 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 5761 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 5821 cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga ttacaaaatt 5881 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 5941 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg 6001 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc 6061 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt 6121 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc 6181 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 6241 ttgtcgggga aATCATCGTC CTTTCCTtgg ctgctcgcct gtgttgccac ctggattctg 6301 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc 6361 ggcctgctgc cggctctgcg gcctcttccg cgtctTcgcc ttcgccctca gacgagtcgg 6421 atctcccttt ggGccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc 6481 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca 6541 acgaaGataa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc 6601 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg 6661 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag 6721 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca 6781 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc 6841 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 6901 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta 6961 gctaTCCCGC CCCTAACTCC GCCCAtcccg cccctaactc cgcccagttc cgcccattct 7021 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct 7081 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa 7141 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 7201 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 7261 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 7321 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 7381 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7441 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7501 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7561 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7621 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7681 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7741 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7801 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7861 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7921 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7981 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 8041 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 8101 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 8161 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 8221 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 8281 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 8341 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8401 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8461 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8521 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8581 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 8641 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 8701 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 8761 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 8821 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 8881 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 8941 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 9001 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 9061 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 9121 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 9181 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 9241 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 9301 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 9361 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 9421 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 9481 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 9541 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 9601 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 9661 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 9721 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.